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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human HLA class I histocompatibility antigen, Cw-7 alpha chain (HLA-C), partial CSB-YP320739HU
CSB-EP320739HU
CSB-BP320739HU
CSB-MP320739HU
CSB-EP320739HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Ferredoxin-like protein (fixX) CSB-YP320740BVW
CSB-EP320740BVW
CSB-BP320740BVW
CSB-MP320740BVW
CSB-EP320740BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Accessory gland-specific peptide 26Ab (Acp26Ab) CSB-YP320741DLU
CSB-EP320741DLU
CSB-BP320741DLU
CSB-MP320741DLU
CSB-EP320741DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutamine transport ATP-binding protein GlnQ (glnQ) CSB-YP320742ENV
CSB-EP320742ENV
CSB-BP320742ENV
CSB-MP320742ENV
CSB-EP320742ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein PET122, mitochondrial (PET122) CSB-YP320743SVG
CSB-EP320743SVG
CSB-BP320743SVG
CSB-MP320743SVG
CSB-EP320743SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA replicase polyprotein, partial CSB-YP320744TJO
CSB-EP320744TJO
CSB-BP320744TJO
CSB-MP320744TJO
CSB-EP320744TJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Histidinol-phosphate aminotransferase (hisC) CSB-YP320745SXB
CSB-EP320745SXB
CSB-BP320745SXB
CSB-MP320745SXB
CSB-EP320745SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein ovarian tumor locus (otu), partial CSB-YP320746DLU
CSB-EP320746DLU
CSB-BP320746DLU
CSB-MP320746DLU
CSB-EP320746DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Retrovirus-related Pol polyprotein from transposon 412 (POL), partial CSB-YP320747DLU
CSB-EP320747DLU
CSB-BP320747DLU
CSB-MP320747DLU
CSB-EP320747DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Retrovirus-related Pol polyprotein (Pol) CSB-YP320748MO
CSB-EP320748MO
CSB-BP320748MO
CSB-MP320748MO
CSB-EP320748MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Retrovirus-related Env polyprotein from transposon gypsy (env), partial CSB-YP320749DLU
CSB-EP320749DLU
CSB-BP320749DLU
CSB-MP320749DLU
CSB-EP320749DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus E serotype 4 Early E1A 28 kDa protein CSB-YP320750HIQ
CSB-EP320750HIQ
CSB-BP320750HIQ
CSB-MP320750HIQ
CSB-EP320750HIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ambrosia trifida Pollen allergen Amb t 5 CSB-YP320751AIT
CSB-EP320751AIT
CSB-BP320751AIT
CSB-MP320751AIT
CSB-EP320751AIT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myeloproliferative sarcoma virus Serine/threonine-protein kinase-transforming protein mos (V-MOS) CSB-YP320752MMC
CSB-EP320752MMC
CSB-BP320752MMC
CSB-MP320752MMC
CSB-EP320752MMC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage phi105 Uncharacterized immunity region protein 2 CSB-YP320753BBA
CSB-EP320753BBA
CSB-BP320753BBA
CSB-MP320753BBA
CSB-EP320753BBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage phi105 Putative uncharacterized immunity region protein 9 CSB-YP320754BBA
CSB-EP320754BBA
CSB-BP320754BBA
CSB-MP320754BBA
CSB-EP320754BBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage phi105 Uncharacterized immunity region protein 12 CSB-YP320755BBA
CSB-EP320755BBA
CSB-BP320755BBA
CSB-MP320755BBA
CSB-EP320755BBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage PA-2 Endopeptidase (Rz), partial CSB-YP320756EDJ
CSB-EP320756EDJ
CSB-BP320756EDJ
CSB-MP320756EDJ
CSB-EP320756EDJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Protein fixB (fixB) CSB-YP320757BVW
CSB-EP320757BVW
CSB-BP320757BVW
CSB-MP320757BVW
CSB-EP320757BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laticauda colubrina Short neurotoxin D CSB-YP320758LCZ
CSB-EP320758LCZ
CSB-BP320758LCZ
CSB-MP320758LCZ
CSB-EP320758LCZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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