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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Nicotiana tabacum Photosystem II reaction center protein T (psbT), partial CSB-YP320944NHE
CSB-EP320944NHE
CSB-BP320944NHE
CSB-MP320944NHE
CSB-EP320944NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Photosystem I reaction center subunit IX (psaJ), partial CSB-YP320945OFG
CSB-EP320945OFG
CSB-BP320945OFG
CSB-MP320945OFG
CSB-EP320945OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP320946MCB1
CSB-EP320946MCB1
CSB-BP320946MCB1
CSB-MP320946MCB1
CSB-EP320946MCB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Chloroplast envelope membrane protein (cemA), partial CSB-YP320947MCB1
CSB-EP320947MCB1
CSB-BP320947MCB1
CSB-MP320947MCB1
CSB-EP320947MCB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Putative membrane protein ycf1 (ycf1), partial CSB-YP320948NHE
CSB-EP320948NHE
CSB-BP320948NHE
CSB-MP320948NHE
CSB-EP320948NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea 50S ribosomal protein L36, chloroplastic (rpl36) CSB-YP320949FKI
CSB-EP320949FKI
CSB-BP320949FKI
CSB-MP320949FKI
CSB-EP320949FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nodulation protein D 2 (nodD2) CSB-YP320950BLH
CSB-EP320950BLH
CSB-BP320950BLH
CSB-MP320950BLH
CSB-EP320950BLH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus vulcanus Photosystem II reaction center protein L (psbL), partial CSB-YP320951TNI
CSB-EP320951TNI
CSB-BP320951TNI
CSB-MP320951TNI
CSB-EP320951TNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposase for insertion sequence element IS231A CSB-YP320952BCW
CSB-EP320952BCW
CSB-BP320952BCW
CSB-MP320952BCW
CSB-EP320952BCW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Non-histone chromosomal protein HMG-14B (HMG14) CSB-YP320954CH
CSB-EP320954CH
CSB-BP320954CH
CSB-MP320954CH
CSB-EP320954CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aplysia californica Neuroactive polyprotein R15 CSB-YP320955DNM
CSB-EP320955DNM
CSB-BP320955DNM
CSB-MP320955DNM
CSB-EP320955DNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein suppressor of white apricot (su (w[a])), partial CSB-YP320956DLU
CSB-EP320956DLU
CSB-BP320956DLU
CSB-MP320956DLU
CSB-EP320956DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Oxygen-evolving enhancer protein 2, chloroplastic (PSBP) CSB-YP320957FKI
CSB-EP320957FKI
CSB-BP320957FKI
CSB-MP320957FKI
CSB-EP320957FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus vulcanus Photosystem II reaction center protein M (psbM), partial CSB-YP320958TNI
CSB-EP320958TNI
CSB-BP320958TNI
CSB-MP320958TNI
CSB-EP320958TNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Chlorophyll a-b binding protein 2, chloroplastic (CAB2R), partial CSB-YP320959OFG1
CSB-EP320959OFG1
CSB-BP320959OFG1
CSB-MP320959OFG1
CSB-EP320959OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila pseudoobscura pseudoobscura Period circadian protein (per), partial CSB-YP320960DME
CSB-EP320960DME
CSB-BP320960DME
CSB-MP320960DME
CSB-EP320960DME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Photosystem I reaction center subunit II, chloroplastic (psaD) CSB-YP320961FKI
CSB-EP320961FKI
CSB-BP320961FKI
CSB-MP320961FKI
CSB-EP320961FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Photosystem I reaction center subunit V, chloroplastic (PSAG), partial CSB-YP320962FKI1
CSB-EP320962FKI1
CSB-BP320962FKI1
CSB-MP320962FKI1
CSB-EP320962FKI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Photosystem II reaction center protein H (psbH), partial CSB-YP320963HWQ1
CSB-EP320963HWQ1
CSB-BP320963HWQ1
CSB-MP320963HWQ1
CSB-EP320963HWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Copper resistance protein A (copA), partial CSB-YP320964PWG
CSB-EP320964PWG
CSB-BP320964PWG
CSB-MP320964PWG
CSB-EP320964PWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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