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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Paralithodes camtschaticus Collagenolytic protease 36 kDa C CSB-YP324084PCF
CSB-EP324084PCF
CSB-BP324084PCF
CSB-MP324084PCF
CSB-EP324084PCF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Boophilus microplus Glycoprotein antigen BM86, partial CSB-YP324085BTV
CSB-EP324085BTV
CSB-BP324085BTV
CSB-MP324085BTV
CSB-EP324085BTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Ig lambda chain C region CSB-YP324086CH
CSB-EP324086CH
CSB-BP324086CH
CSB-MP324086CH
CSB-EP324086CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. C-phycoerythrin beta chain (cpeB) CSB-YP324087SSN
CSB-EP324087SSN
CSB-BP324087SSN
CSB-MP324087SSN
CSB-EP324087SSN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuticle collagen rol-6 (rol-6) CSB-YP324088CXY
CSB-EP324088CXY
CSB-BP324088CXY
CSB-MP324088CXY
CSB-EP324088CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell surface receptor daf-1 (daf-1), partial CSB-YP324089CXY
CSB-EP324089CXY
CSB-BP324089CXY
CSB-MP324089CXY
CSB-EP324089CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antheraea polyphemus Pheromone-binding protein CSB-YP324090BZY
CSB-EP324090BZY
CSB-BP324090BZY
CSB-MP324090BZY
CSB-EP324090BZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Early nodulin-12A (ENOD12A) CSB-YP324091EWE
CSB-EP324091EWE
CSB-BP324091EWE
CSB-MP324091EWE
CSB-EP324091EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Interleukin-11 (IL11) CSB-YP324092MOV
CSB-BP324092MOV
CSB-MP324092MOV
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T3 Gene 1.1 protein (1.1) CSB-YP324093EDY
CSB-EP324093EDY
CSB-BP324093EDY
CSB-MP324093EDY
CSB-EP324093EDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Putative serine proteinase inhibitor 2 homolog first part (B13R) CSB-YP324094VAA
CSB-EP324094VAA
CSB-BP324094VAA
CSB-MP324094VAA
CSB-EP324094VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Thioredoxin-2 (trxB) CSB-YP324095NHR
CSB-EP324095NHR
CSB-BP324095NHR
CSB-MP324095NHR
CSB-EP324095NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Chlorophyll a-b binding protein, chloroplastic, partial CSB-YP324096EVD1
CSB-EP324096EVD1
CSB-BP324096EVD1
CSB-MP324096EVD1
CSB-EP324096EVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Rev (rev) CSB-YP324097HKS
CSB-EP324097HKS
CSB-BP324097HKS
CSB-MP324097HKS
CSB-EP324097HKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Gag-Pol polyprotein (gag-pol), partial CSB-YP324098HMB
CSB-EP324098HMB
CSB-BP324098HMB
CSB-MP324098HMB
CSB-EP324098HMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Vpx (vpx) CSB-YP324099HMB
CSB-EP324099HMB
CSB-BP324099HMB
CSB-MP324099HMB
CSB-EP324099HMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Vpr (vpr) CSB-YP324100HMB
CSB-EP324100HMB
CSB-BP324100HMB
CSB-MP324100HMB
CSB-EP324100HMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Nef (nef) CSB-YP324101HKZ
CSB-EP324101HKZ
CSB-BP324101HKZ
CSB-MP324101HKZ
CSB-EP324101HKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Gag-Pol polyprotein (gag-pol), partial CSB-YP324102HKZ
CSB-EP324102HKZ
CSB-BP324102HKZ
CSB-MP324102HKZ
CSB-EP324102HKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycolysin (npr) CSB-YP324103SNR
CSB-EP324103SNR
CSB-BP324103SNR
CSB-MP324103SNR
CSB-EP324103SNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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