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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Spiroplasma virus SpV1-R8A2 B Uncharacterized protein ORF1 (ORF1), partial CSB-YP324499SJZ1
CSB-EP324499SJZ1
CSB-BP324499SJZ1
CSB-MP324499SJZ1
CSB-EP324499SJZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus SpV1-R8A2 B Uncharacterized protein ORF10 (ORF10), partial CSB-YP324500SJZ1
CSB-EP324500SJZ1
CSB-BP324500SJZ1
CSB-MP324500SJZ1
CSB-EP324500SJZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowlpox virus Protein FPV127 (FPV127, fp9.127) CSB-YP324501FEY
CSB-EP324501FEY
CSB-BP324501FEY
CSB-MP324501FEY
CSB-EP324501FEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteriocin UviB (uviB) CSB-YP324503CMB
CSB-EP324503CMB
CSB-BP324503CMB
CSB-MP324503CMB
CSB-EP324503CMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artichoke mottled crinkle virus RNA silencing suppressor p19 (ORF4) CSB-YP324504AOX
CSB-EP324504AOX
CSB-BP324504AOX
CSB-MP324504AOX
CSB-EP324504AOX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hololena curta Mu-agatoxin-Hc1c CSB-YP324505HHN
CSB-EP324505HHN
CSB-BP324505HHN
CSB-MP324505HHN
CSB-EP324505HHN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig SLA class II histocompatibility antigen, DQ haplotype C alpha chain, partial CSB-YP324506PI
CSB-EP324506PI
CSB-BP324506PI
CSB-MP324506PI
CSB-EP324506PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Actin-7 (SAC7) CSB-YP324507GGV
CSB-EP324507GGV
CSB-BP324507GGV
CSB-MP324507GGV
CSB-EP324507GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Apocytochrome f (petA), partial CSB-YP324508FKI1
CSB-EP324508FKI1
CSB-BP324508FKI1
CSB-MP324508FKI1
CSB-EP324508FKI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesembryanthemum crystallinum Ribulose bisphosphate carboxylase small chain 1, chloroplastic (RBCS-1) CSB-YP324510MRC
CSB-EP324510MRC
CSB-BP324510MRC
CSB-MP324510MRC
CSB-EP324510MRC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus keta Vasotocin-neurophysin VT 1 CSB-YP324511OBG
CSB-EP324511OBG
CSB-BP324511OBG
CSB-MP324511OBG
CSB-EP324511OBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Glycine cleavage system H protein, mitochondrial (GDCSH) CSB-YP324512EWE
CSB-EP324512EWE
CSB-BP324512EWE
CSB-MP324512EWE
CSB-EP324512EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Myoblast determination protein 1 homolog B (myod1-b) CSB-YP324513XBE
CSB-EP324513XBE
CSB-BP324513XBE
CSB-MP324513XBE
CSB-EP324513XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline immunodeficiency virus Envelope glycoprotein gp150 (env), partial CSB-YP324515FAK
CSB-EP324515FAK
CSB-BP324515FAK
CSB-MP324515FAK
CSB-EP324515FAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Beta-amylase (BMY1), partial CSB-YP324516HWQ
CSB-EP324516HWQ
CSB-BP324516HWQ
CSB-MP324516HWQ
CSB-EP324516HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polyandrocarpa misakiensis Lectin CSB-YP324517POO
CSB-EP324517POO
CSB-BP324517POO
CSB-MP324517POO
CSB-EP324517POO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga maritima L-lactate dehydrogenase (ldh) CSB-YP324518TNJ
CSB-EP324518TNJ
CSB-BP324518TNJ
CSB-MP324518TNJ
CSB-EP324518TNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Urease subunit gamma (ureA) CSB-YP324519PSP
CSB-EP324519PSP
CSB-BP324519PSP
CSB-MP324519PSP
CSB-EP324519PSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetabularia cliftonii Ribulose bisphosphate carboxylase small chain 4, chloroplastic (RBCS-4) CSB-YP324520AAW
CSB-EP324520AAW
CSB-BP324520AAW
CSB-MP324520AAW
CSB-EP324520AAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetabularia acetabulum Ribulose bisphosphate carboxylase small chain 4, chloroplastic (RBCS-4) CSB-YP324521AAV
CSB-EP324521AAV
CSB-BP324521AAV
CSB-MP324521AAV
CSB-EP324521AAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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