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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae ATP-dependent RNA helicase DBP3 (DBP3), partial CSB-YP324043SVG
CSB-EP324043SVG
CSB-BP324043SVG
CSB-MP324043SVG
CSB-EP324043SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Protein ndvB (ndvB), partial CSB-YP324045RKU
CSB-EP324045RKU
CSB-BP324045RKU
CSB-MP324045RKU
CSB-EP324045RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein claret segregational (ncd), partial CSB-YP324046DLU
CSB-EP324046DLU
CSB-BP324046DLU
CSB-MP324046DLU
CSB-EP324046DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Choline kinase (CKI1), partial CSB-YP324047SVG
CSB-EP324047SVG
CSB-BP324047SVG
CSB-MP324047SVG
CSB-EP324047SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Flagellar FliJ protein (fliJ) CSB-YP324048BRJ
CSB-EP324048BRJ
CSB-BP324048BRJ
CSB-MP324048BRJ
CSB-EP324048BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus DNA ligase (LIG), partial CSB-YP324049VAA
CSB-EP324049VAA
CSB-BP324049VAA
CSB-MP324049VAA
CSB-EP324049VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein H2 (H2R), partial CSB-YP324050VAA1
CSB-EP324050VAA1
CSB-BP324050VAA1
CSB-MP324050VAA1
CSB-EP324050VAA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Core protease I7 (I7L) CSB-YP324051VAA
CSB-EP324051VAA
CSB-BP324051VAA
CSB-MP324051VAA
CSB-EP324051VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium L-Threonine dehydratase biosynthetic IlvA (ilvA), partial CSB-YP324052SXB
CSB-EP324052SXB
CSB-BP324052SXB
CSB-MP324052SXB
CSB-EP324052SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 14.1 kDa protein (A ORF A) CSB-YP324053VAA
CSB-EP324053VAA
CSB-BP324053VAA
CSB-MP324053VAA
CSB-EP324053VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 8.4 kDa protein (A ORF F) CSB-YP324054VAA
CSB-EP324054VAA
CSB-BP324054VAA
CSB-MP324054VAA
CSB-EP324054VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.5 kDa protein (A ORF K) CSB-YP324055VAA
CSB-EP324055VAA
CSB-BP324055VAA
CSB-MP324055VAA
CSB-EP324055VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chitinase A1 (chiA1), partial CSB-YP324056BAN
CSB-EP324056BAN
CSB-BP324056BAN
CSB-MP324056BAN
CSB-EP324056BAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 10.8 kDa protein (B ORF C) CSB-YP324057VAA
CSB-EP324057VAA
CSB-BP324057VAA
CSB-MP324057VAA
CSB-EP324057VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.6 kDa protein (B ORF I) CSB-YP324058VAA
CSB-EP324058VAA
CSB-BP324058VAA
CSB-MP324058VAA
CSB-EP324058VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 11.9 kDa protein (E ORF B) CSB-YP324059VAA
CSB-EP324059VAA
CSB-BP324059VAA
CSB-MP324059VAA
CSB-EP324059VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.6 kDa protein (E ORF E) CSB-YP324060VAA
CSB-EP324060VAA
CSB-BP324060VAA
CSB-MP324060VAA
CSB-EP324060VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.1 kDa protein (F ORF A) CSB-YP324061VAA
CSB-EP324061VAA
CSB-BP324061VAA
CSB-MP324061VAA
CSB-EP324061VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.8 kDa protein (F ORF E) CSB-YP324062VAA
CSB-EP324062VAA
CSB-BP324062VAA
CSB-MP324062VAA
CSB-EP324062VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Anthranilate phosphoribosyltransferase (trpD) CSB-YP324063EZX
CSB-EP324063EZX
CSB-BP324063EZX
CSB-MP324063EZX
CSB-EP324063EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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