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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis UPF0735 ACT domain-containing protein yszB (yszB) CSB-YP324144BRJ
CSB-EP324144BRJ
CSB-BP324144BRJ
CSB-MP324144BRJ
CSB-EP324144BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium sp. 12-alpha-hydroxysteroid dehydrogenase CSB-YP324146CMH
CSB-EP324146CMH
CSB-BP324146CMH
CSB-MP324146CMH
CSB-EP324146CMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protochlorophyllide reductase B, chloroplastic (PORB) CSB-YP324147DOA
CSB-EP324147DOA
CSB-BP324147DOA
CSB-MP324147DOA
CSB-EP324147DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30 kDa neutral phosphatase, partial CSB-YP324148FKZ
CSB-BP324148FKZ
CSB-MP324148FKZ
CSB-EP324148FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Endochitinase A2 (CHI2) CSB-YP324149EWE
CSB-EP324149EWE
CSB-BP324149EWE
CSB-MP324149EWE
CSB-EP324149EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Broadhaven virus Outer capsid protein VP5 (S5) CSB-YP324150BME
CSB-EP324150BME
CSB-BP324150BME
CSB-MP324150BME
CSB-EP324150BME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 23S rRNA methylase leader peptide (ermC) CSB-YP324151FMW
CSB-EP324151FMW
CSB-BP324151FMW
CSB-MP324151FMW
CSB-EP324151FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Chaperonin 60 subunit alpha 1, chloroplastic (CPN60A1), partial CSB-YP324152DOA
CSB-EP324152DOA
CSB-BP324152DOA
CSB-MP324152DOA
CSB-EP324152DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica napus RuBisCO large subunit-binding protein subunit alpha, chloroplastic, partial CSB-YP324153BWD
CSB-EP324153BWD
CSB-BP324153BWD
CSB-MP324153BWD
CSB-EP324153BWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Tir chaperone (cesT) CSB-YP324154EOB
CSB-EP324154EOB
CSB-BP324154EOB
CSB-MP324154EOB
CSB-EP324154EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major antigen (OVT1), partial CSB-YP324155OEG
CSB-EP324155OEG
CSB-BP324155OEG
CSB-MP324155OEG
CSB-EP324155OEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Immunodominant antigen Ov33-3 CSB-YP324156OEG
CSB-EP324156OEG
CSB-BP324156OEG
CSB-MP324156OEG
CSB-EP324156OEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. Pesticidal crystal protein cry2Ab (cry2Ab) CSB-YP324157BDC
CSB-BP324157BDC
CSB-MP324157BDC
CSB-EP324157BDC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polyorchis penicillatus Pol-RFamide neuropeptides CSB-YP324158POS
CSB-EP324158POS
CSB-BP324158POS
CSB-MP324158POS
CSB-EP324158POS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cyclin-dependent kinase inhibitor FAR1 (FAR1), partial CSB-YP324159SVG
CSB-EP324159SVG
CSB-BP324159SVG
CSB-MP324159SVG
CSB-EP324159SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mumps virus Nucleoprotein (N), partial CSB-YP324160MJM
CSB-EP324160MJM
CSB-BP324160MJM
CSB-MP324160MJM
CSB-EP324160MJM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Autographa californica nuclear polyhedrosis virus Uncharacterized 24.8 kDa protein in FGF-VUBI intergenic region CSB-YP324161ARA
CSB-EP324161ARA
CSB-BP324161ARA
CSB-MP324161ARA
CSB-EP324161ARA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Autographa californica nuclear polyhedrosis virus Uncharacterized 25.3 kDa protein in 39K-P43 intergenic region CSB-YP324162ARA
CSB-EP324162ARA
CSB-BP324162ARA
CSB-MP324162ARA
CSB-EP324162ARA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Flagellum biosynthesis repressor protein flbT (flbT) CSB-YP324163DQO
CSB-EP324163DQO
CSB-BP324163DQO
CSB-MP324163DQO
CSB-EP324163DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sonchus yellow net virus Phosphoprotein (P) CSB-YP324164SIV
CSB-EP324164SIV
CSB-BP324164SIV
CSB-MP324164SIV
CSB-EP324164SIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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