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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Uncharacterized protein in traD-traI intergenic region CSB-YP324704ENV
CSB-EP324704ENV
CSB-BP324704ENV
CSB-MP324704ENV
CSB-EP324704ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.5 kDa protein CSB-YP324705VAI
CSB-EP324705VAI
CSB-BP324705VAI
CSB-MP324705VAI
CSB-EP324705VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ybdZ (ybdZ) CSB-YP324706ENV
CSB-EP324706ENV
CSB-BP324706ENV
CSB-MP324706ENV
CSB-EP324706ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis ER lumen protein retaining receptor (ERD2), partial CSB-YP324707KBK1
CSB-EP324707KBK1
CSB-BP324707KBK1
CSB-MP324707KBK1
CSB-EP324707KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tarsius syrichta Hemoglobin subunit gamma (HBG) CSB-YP324708TLJ
CSB-EP324708TLJ
CSB-BP324708TLJ
CSB-MP324708TLJ
CSB-EP324708TLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine transmissible gastroenteritis coronavirus Spike glycoprotein (S), partial CSB-YP324709PQF
CSB-EP324709PQF
CSB-BP324709PQF
CSB-MP324709PQF
CSB-EP324709PQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog DLA class I histocompatibility antigen, A9/A9 alpha chain, partial CSB-YP324710DO
CSB-EP324710DO
CSB-BP324710DO
CSB-MP324710DO
CSB-EP324710DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein traU (traU) CSB-YP324711ENV
CSB-EP324711ENV
CSB-BP324711ENV
CSB-MP324711ENV
CSB-EP324711ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus awamori Anthranilate synthase component 2 (trpC), partial CSB-YP324712APL
CSB-EP324712APL
CSB-BP324712APL
CSB-MP324712APL
CSB-EP324712APL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Synaptobrevin (Syb), partial CSB-YP324713DLU
CSB-EP324713DLU
CSB-BP324713DLU
CSB-MP324713DLU
CSB-EP324713DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Protein hesA (hesA) CSB-YP324714NHR
CSB-EP324714NHR
CSB-BP324714NHR
CSB-MP324714NHR
CSB-EP324714NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowlpox virus Protein I5 homolog (FPV085), partial CSB-YP324715FEY1
CSB-EP324715FEY1
CSB-BP324715FEY1
CSB-MP324715FEY1
CSB-EP324715FEY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ig heavy chain V region 345 CSB-YP324716MO
CSB-EP324716MO
CSB-BP324716MO
CSB-MP324716MO
CSB-EP324716MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ig heavy chain V region 1B43 CSB-YP324717MO
CSB-EP324717MO
CSB-BP324717MO
CSB-MP324717MO
CSB-EP324717MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Acetylornithine aminotransferase, mitochondrial (ARG8), partial CSB-YP324718SVG
CSB-EP324718SVG
CSB-BP324718SVG
CSB-MP324718SVG
CSB-EP324718SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Envelope glycoprotein B (gB), partial CSB-YP324719EFI
CSB-EP324719EFI
CSB-BP324719EFI
CSB-MP324719EFI
CSB-EP324719EFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti HTH-type transcriptional regulator syrM (syrM) CSB-YP324720RKU
CSB-EP324720RKU
CSB-BP324720RKU
CSB-MP324720RKU
CSB-EP324720RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Ribulose bisphosphate carboxylase small chain A, chloroplastic (RBCS-A) CSB-YP324721OFG
CSB-EP324721OFG
CSB-BP324721OFG
CSB-MP324721OFG
CSB-EP324721OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Blood group Rh (CE) polypeptide (RHCE), partial CSB-YP324722HU
CSB-BP324722HU
CSB-MP324722HU
CSB-EP324722HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfur-rich protein, serovars L1/L3 (srp), partial CSB-YP324723DSA1
CSB-EP324723DSA1
CSB-BP324723DSA1
CSB-MP324723DSA1
CSB-EP324723DSA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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