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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Fructose-bisphosphate aldolase, cytoplasmic isozyme (At4g26520) CSB-YP325243DOA
CSB-EP325243DOA
CSB-BP325243DOA
CSB-MP325243DOA
CSB-EP325243DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Pyruvate kinase, cytosolic isozyme, partial CSB-YP325244FIG
CSB-EP325244FIG
CSB-BP325244FIG
CSB-MP325244FIG
CSB-EP325244FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Heat shock protein SSA4 (SSA4), partial CSB-YP325245SVG
CSB-EP325245SVG
CSB-BP325245SVG
CSB-MP325245SVG
CSB-EP325245SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase KIN3 (KIN3) CSB-YP325246SVG
CSB-EP325246SVG
CSB-BP325246SVG
CSB-MP325246SVG
CSB-EP325246SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein transport protein SEC22 (SEC22), partial CSB-YP325247SVG
CSB-EP325247SVG
CSB-BP325247SVG
CSB-MP325247SVG
CSB-EP325247SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Thioredoxin-1 (TRX1) CSB-YP325248SVG
CSB-EP325248SVG
CSB-BP325248SVG
CSB-MP325248SVG
CSB-EP325248SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flaveria trinervia Pyruvate, phosphate dikinase, chloroplastic (PPDK), partial CSB-YP325249FEU
CSB-EP325249FEU
CSB-BP325249FEU
CSB-MP325249FEU
CSB-EP325249FEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Exocyst complex component SEC15 (SEC15), partial CSB-YP325250SVG
CSB-EP325250SVG
CSB-BP325250SVG
CSB-MP325250SVG
CSB-EP325250SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine adenovirus serotype 1 Hexon-associated protein (PVIII) CSB-YP325251CCK
CSB-EP325251CCK
CSB-BP325251CCK
CSB-MP325251CCK
CSB-EP325251CCK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desiccation-related protein PCC27-45 CSB-YP325252DXS
CSB-EP325252DXS
CSB-BP325252DXS
CSB-MP325252DXS
CSB-EP325252DXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Protein synthesis inhibitor I (RIP30) CSB-YP325253HWQ
CSB-EP325253HWQ
CSB-BP325253HWQ
CSB-MP325253HWQ
CSB-EP325253HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glutamate--tRNA ligase (gltX) CSB-YP325254BRJ
CSB-EP325254BRJ
CSB-BP325254BRJ
CSB-MP325254BRJ
CSB-EP325254BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphoenolpyruvate carboxykinase [ATP] (pckA) CSB-YP325256ENV
CSB-EP325256ENV
CSB-BP325256ENV
CSB-MP325256ENV
CSB-EP325256ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Listeriolysin regulatory protein (prfA) CSB-YP325257LPY
CSB-EP325257LPY
CSB-BP325257LPY
CSB-MP325257LPY
CSB-EP325257LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces scabies Esterase (estA) CSB-YP325258SPN
CSB-EP325258SPN
CSB-BP325258SPN
CSB-MP325258SPN
CSB-EP325258SPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans ADP-ribosylation factor (ARF1) CSB-YP325259CZD
CSB-EP325259CZD
CSB-BP325259CZD
CSB-MP325259CZD
CSB-EP325259CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mucor circinelloides f. lusitanicus Ras-like protein 2 (RAS2) CSB-YP325260MIW
CSB-EP325260MIW
CSB-BP325260MIW
CSB-MP325260MIW
CSB-EP325260MIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Cystatin-related protein 1 (Andpro) CSB-YP325261RA
CSB-EP325261RA
CSB-BP325261RA
CSB-MP325261RA
CSB-EP325261RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Poa pratensis Pollen allergen KBG 41 CSB-YP325262PNP
CSB-EP325262PNP
CSB-BP325262PNP
CSB-MP325262PNP
CSB-EP325262PNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cladosporium fulvum Race-specific elicitor A9 (AVR9) CSB-YP325263DTS
CSB-EP325263DTS
CSB-BP325263DTS
CSB-MP325263DTS
CSB-EP325263DTS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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