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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Panolis flammea multiple nucleocapsid polyhedrosis virus Polyhedrin (PH) CSB-YP325222PBC
CSB-EP325222PBC
CSB-BP325222PBC
CSB-MP325222PBC
CSB-EP325222PBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA gyrase subunit B (gyrB), partial CSB-YP325223NFZ
CSB-EP325223NFZ
CSB-BP325223NFZ
CSB-MP325223NFZ
CSB-EP325223NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Heat shock factor protein (HSF), partial CSB-YP325224KBK
CSB-EP325224KBK
CSB-BP325224KBK
CSB-MP325224KBK
CSB-EP325224KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Discopyge ommata Ras-related protein O-RAL CSB-YP325225DES
CSB-EP325225DES
CSB-BP325225DES
CSB-MP325225DES
CSB-EP325225DES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Malate dehydrogenase, cytoplasmic (MDH2) CSB-YP325226SVG
CSB-EP325226SVG
CSB-BP325226SVG
CSB-MP325226SVG
CSB-EP325226SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATPase expression protein 2, mitochondrial (AEP2), partial CSB-YP325227SVG
CSB-EP325227SVG
CSB-BP325227SVG
CSB-MP325227SVG
CSB-EP325227SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Glucose-repressible gene protein (grg-1) CSB-YP325229NHA
CSB-EP325229NHA
CSB-BP325229NHA
CSB-MP325229NHA
CSB-EP325229NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhesus papillomavirus type 1 Replication protein E1 (E1), partial CSB-YP325230RCO
CSB-EP325230RCO
CSB-BP325230RCO
CSB-MP325230RCO
CSB-EP325230RCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein E2 (E2) CSB-YP325231HOD
CSB-EP325231HOD
CSB-BP325231HOD
CSB-MP325231HOD
CSB-EP325231HOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein E7 (E7) CSB-YP325232HOD
CSB-EP325232HOD
CSB-BP325232HOD
CSB-MP325232HOD
CSB-EP325232HOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major capsid protein L1 (L1), partial CSB-YP325233HOD
CSB-EP325233HOD
CSB-BP325233HOD
CSB-MP325233HOD
CSB-EP325233HOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Minor capsid protein L2 (L2) CSB-YP325234HOD
CSB-EP325234HOD
CSB-BP325234HOD
CSB-MP325234HOD
CSB-EP325234HOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Movement and silencing protein TGBp1 (ORF2) CSB-YP325235FCN
CSB-EP325235FCN
CSB-BP325235FCN
CSB-MP325235FCN
CSB-EP325235FCN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Movement protein TGB2 (ORF3), partial CSB-YP325236FCN1
CSB-EP325236FCN1
CSB-BP325236FCN1
CSB-MP325236FCN1
CSB-EP325236FCN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Infectious pancreatic necrosis virus RNA-directed RNA polymerase (VP1), partial CSB-YP325237IAT
CSB-EP325237IAT
CSB-BP325237IAT
CSB-MP325237IAT
CSB-EP325237IAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flaveria trinervia NADP-dependent malic enzyme, chloroplastic (MOD1), partial CSB-YP325238FEU
CSB-EP325238FEU
CSB-BP325238FEU
CSB-MP325238FEU
CSB-EP325238FEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helianthus annuus Anther-specific protein SF2 CSB-YP325239HUO
CSB-EP325239HUO
CSB-BP325239HUO
CSB-MP325239HUO
CSB-EP325239HUO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP325240ENV
CSB-EP325240ENV
CSB-BP325240ENV
CSB-MP325240ENV
CSB-EP325240ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized cox1 intron-1 45.6 kDa protein (SPMIT.02) CSB-YP325241SXV
CSB-EP325241SXV
CSB-BP325241SXV
CSB-MP325241SXV
CSB-EP325241SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Protein phosphatase 1 regulatory subunit SDS22 (sds22) CSB-YP325242SXV
CSB-EP325242SXV
CSB-BP325242SXV
CSB-MP325242SXV
CSB-EP325242SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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