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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Orgyia pseudotsugata multicapsid polyhedrosis virus Polyhedral envelope protein (ORF129) CSB-YP325507OEU
CSB-EP325507OEU
CSB-BP325507OEU
CSB-MP325507OEU
CSB-EP325507OEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Malate dehydrogenase, mitochondrial (MDH1) CSB-YP325508SVG
CSB-EP325508SVG
CSB-BP325508SVG
CSB-MP325508SVG
CSB-EP325508SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato leafroll virus Major capsid protein (ORF3) CSB-YP325509PQW
CSB-EP325509PQW
CSB-BP325509PQW
CSB-MP325509PQW
CSB-EP325509PQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato virus M Coat protein CSB-YP325510PRA
CSB-EP325510PRA
CSB-BP325510PRA
CSB-MP325510PRA
CSB-EP325510PRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas moewusii Ribulose bisphosphate carboxylase small chain, chloroplastic (RBCS) CSB-YP325511CIX
CSB-EP325511CIX
CSB-BP325511CIX
CSB-MP325511CIX
CSB-EP325511CIX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase II subunit RPB1-B (TRP5.9), partial CSB-YP325512TQV
CSB-EP325512TQV
CSB-BP325512TQV
CSB-MP325512TQV
CSB-EP325512TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana S-adenosylmethionine synthase 2 (SAM2) CSB-YP325513DOA
CSB-EP325513DOA
CSB-BP325513DOA
CSB-MP325513DOA
CSB-EP325513DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Nitrate reductase [NADH], partial CSB-YP325514ZAX
CSB-EP325514ZAX
CSB-BP325514ZAX
CSB-MP325514ZAX
CSB-EP325514ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Aminoglycoside 6-adenylyltransferase (aadK) CSB-YP325515BRJ
CSB-EP325515BRJ
CSB-BP325515BRJ
CSB-MP325515BRJ
CSB-EP325515BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Encephalomyocarditis virus Genome polyprotein CSB-YP325516EBU
CSB-EP325516EBU
CSB-BP325516EBU
CSB-MP325516EBU
CSB-EP325516EBU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 24 kDa flagellin CSB-YP325517MFD
CSB-EP325517MFD
CSB-BP325517MFD
CSB-MP325517MFD
CSB-EP325517MFD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorghum bicolor Malate dehydrogenase [NADP] 1, chloroplastic CSB-YP325518FJS
CSB-EP325518FJS
CSB-BP325518FJS
CSB-MP325518FJS
CSB-EP325518FJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Suid herpesvirus 1 Serine/threonine-protein kinase (PK) CSB-YP325519SRI
CSB-EP325519SRI
CSB-BP325519SRI
CSB-MP325519SRI
CSB-EP325519SRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Nuclear movement protein nudC (nudC) CSB-YP325520EKF
CSB-EP325520EKF
CSB-BP325520EKF
CSB-MP325520EKF
CSB-EP325520EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota EMB-1 protein (EMB-1) CSB-YP325521DIR
CSB-EP325521DIR
CSB-BP325521DIR
CSB-MP325521DIR
CSB-EP325521DIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Opsin Rh3 (Rh3) CSB-YP325522DMP
CSB-EP325522DMP
CSB-BP325522DMP
CSB-MP325522DMP
CSB-EP325522DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aplysia fasciata Abdominal ganglion neuropeptide R3-14 CSB-YP325523AMM
CSB-EP325523AMM
CSB-BP325523AMM
CSB-MP325523AMM
CSB-EP325523AMM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Outer capsid glycoprotein VP7 CSB-YP325524RFU
CSB-BP325524RFU
CSB-MP325524RFU
CSB-EP325524RFU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant X polypeptide (X) CSB-YP325526ENL
CSB-EP325526ENL
CSB-BP325526ENL
CSB-MP325526ENL
CSB-EP325526ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Catalase-peroxidase (katG), partial CSB-YP325527SXB
CSB-EP325527SXB
CSB-BP325527SXB
CSB-MP325527SXB
CSB-EP325527SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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