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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coprinus congregatus Ubiquitin CSB-YP325710CPQ
CSB-EP325710CPQ
CSB-BP325710CPQ
CSB-MP325710CPQ
CSB-EP325710CPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clypeaster japonicus Sperm-activating peptide SAP-b CSB-YP325711CMU
CSB-EP325711CMU
CSB-BP325711CMU
CSB-MP325711CMU
CSB-EP325711CMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Comamonas testosteroni 3-beta-hydroxysteroid dehydrogenase CSB-YP325712CNN
CSB-EP325712CNN
CSB-BP325712CNN
CSB-MP325712CNN
CSB-EP325712CNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus A serotype 12 Hexon protein (PII), partial CSB-YP325713HIB
CSB-EP325713HIB
CSB-BP325713HIB
CSB-MP325713HIB
CSB-EP325713HIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkanal monooxygenase alpha chain (luxA) CSB-YP325714VDZ
CSB-EP325714VDZ
CSB-BP325714VDZ
CSB-MP325714VDZ
CSB-EP325714VDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carbon monoxide dehydrogenase large chain (cutL) CSB-YP325715PUE
CSB-EP325715PUE
CSB-BP325715PUE
CSB-MP325715PUE
CSB-EP325715PUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus necator Phosphoribulokinase, plasmid (cfxP) CSB-YP325716DZQ
CSB-EP325716DZQ
CSB-BP325716DZQ
CSB-MP325716DZQ
CSB-EP325716DZQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Hydrogenase 1 maturation protease (hyaD) CSB-YP325717ENV
CSB-EP325717ENV
CSB-BP325717ENV
CSB-MP325717ENV
CSB-EP325717ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African cassava mosaic virus Transcriptional activator protein (AC2, AL2) CSB-YP325718ADX
CSB-EP325718ADX
CSB-BP325718ADX
CSB-MP325718ADX
CSB-EP325718ADX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beet curly top virus Capsid protein (V1) CSB-YP325719BFX
CSB-EP325719BFX
CSB-BP325719BFX
CSB-MP325719BFX
CSB-EP325719BFX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beet curly top virus Replication-associated protein (C1) CSB-YP325720BFX
CSB-EP325720BFX
CSB-BP325720BFX
CSB-MP325720BFX
CSB-EP325720BFX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant BK polyomavirus Agnoprotein, partial CSB-YP325721BGZ
CSB-EP325721BGZ
CSB-BP325721BGZ
CSB-MP325721BGZ
CSB-EP325721BGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halobacterium salinarum 50S ribosomal protein L22P (rpl22p) CSB-YP325722HTM
CSB-EP325722HTM
CSB-BP325722HTM
CSB-MP325722HTM
CSB-EP325722HTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Fe (3+) dicitrate transport system permease protein fecD, partial CSB-YP325723ENV
CSB-EP325723ENV
CSB-BP325723ENV
CSB-MP325723ENV
CSB-EP325723ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Narcissus mosaic virus Uncharacterized 10 kDa protein CSB-YP325724NAR
CSB-EP325724NAR
CSB-BP325724NAR
CSB-MP325724NAR
CSB-EP325724NAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Superoxide dismutase [Cu-Zn] B (sod1-b) CSB-YP325725XBE
CSB-EP325725XBE
CSB-BP325725XBE
CSB-MP325725XBE
CSB-EP325725XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis 60S ribosomal protein L5-B (rpl5-b) CSB-YP325726XBE
CSB-EP325726XBE
CSB-BP325726XBE
CSB-MP325726XBE
CSB-EP325726XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus C serotype 2 Early E3B 10.4 kDa protein, partial CSB-YP325727HIK
CSB-EP325727HIK
CSB-BP325727HIK
CSB-MP325727HIK
CSB-EP325727HIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Homeobox protein CHOX-7 (CHOX-7) CSB-YP325728CH
CSB-EP325728CH
CSB-BP325728CH
CSB-MP325728CH
CSB-EP325728CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptonychotes weddelli Hemoglobin subunit alpha-1/2 CSB-YP325729LEP
CSB-EP325729LEP
CSB-BP325729LEP
CSB-MP325729LEP
CSB-EP325729LEP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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