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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Hydrogenase isoenzymes formation protein hypD (hypD) CSB-YP326123ENV
CSB-EP326123ENV
CSB-BP326123ENV
CSB-MP326123ENV
CSB-EP326123ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Mrr restriction system protein (mrr) CSB-YP326124ENV
CSB-EP326124ENV
CSB-BP326124ENV
CSB-MP326124ENV
CSB-EP326124ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Flagellar hook-associated protein 2 (fliD) CSB-YP326125ENV
CSB-EP326125ENV
CSB-BP326125ENV
CSB-MP326125ENV
CSB-EP326125ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP-dependent DNA helicase recG (recG) CSB-YP326126ENV
CSB-BP326126ENV
CSB-MP326126ENV
CSB-EP326126ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sigma factor-binding protein crl (crl) CSB-YP326127ENV
CSB-EP326127ENV
CSB-BP326127ENV
CSB-MP326127ENV
CSB-EP326127ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sucrose porin (scrY) CSB-YP326128SBS
CSB-EP326128SBS
CSB-BP326128SBS
CSB-MP326128SBS
CSB-EP326128SBS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Long-chain-fatty-acid--luciferin-component ligase (luxE) CSB-YP326129VDZ
CSB-EP326129VDZ
CSB-BP326129VDZ
CSB-MP326129VDZ
CSB-EP326129VDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia muridarum 30S ribosomal protein S18 (rpsR) CSB-YP326130CIU
CSB-EP326130CIU
CSB-BP326130CIU
CSB-MP326130CIU
CSB-EP326130CIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Outer membrane porin protein 32 (omp32) CSB-YP326131DBE
CSB-EP326131DBE
CSB-BP326131DBE
CSB-MP326131DBE
CSB-EP326131DBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L5 (rplE) CSB-YP326132TNN
CSB-EP326132TNN
CSB-BP326132TNN
CSB-MP326132TNN
CSB-EP326132TNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S17 (rpsQ) CSB-YP326133TNR
CSB-EP326133TNR
CSB-BP326133TNR
CSB-MP326133TNR
CSB-EP326133TNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Foldase protein prsA (prsA) CSB-YP326134BRJ
CSB-EP326134BRJ
CSB-BP326134BRJ
CSB-MP326134BRJ
CSB-EP326134BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus durissus terrificus Myotoxin-3 CSB-YP326135DYD
CSB-EP326135DYD
CSB-BP326135DYD
CSB-MP326135DYD
CSB-EP326135DYD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zinc finger protein sdc-1 (sdc-1), partial CSB-YP326136CXY
CSB-EP326136CXY
CSB-BP326136CXY
CSB-MP326136CXY
CSB-EP326136CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Probable serine/threonine-protein kinase B12 (VACWR194) CSB-YP326137VAI
CSB-EP326137VAI
CSB-BP326137VAI
CSB-MP326137VAI
CSB-EP326137VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Peptidyl-prolyl cis-trans isomerase B (Ppib) CSB-YP326138MO
CSB-EP326138MO
CSB-BP326138MO
CSB-MP326138MO
CSB-EP326138MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Envelope glycoprotein E (gE), partial CSB-YP326139EFN1
CSB-EP326139EFN1
CSB-BP326139EFN1
CSB-MP326139EFN1
CSB-EP326139EFN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyoscyamus niger Hyoscyamine 6-dioxygenase (H6H) CSB-YP326140HYA
CSB-EP326140HYA
CSB-BP326140HYA
CSB-MP326140HYA
CSB-EP326140HYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens 3-isopropylmalate dehydrogenase (leuB) CSB-YP326141AYS
CSB-EP326141AYS
CSB-BP326141AYS
CSB-MP326141AYS
CSB-EP326141AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 4 Protein UL24 homolog (UL24) CSB-YP326142EFR
CSB-EP326142EFR
CSB-BP326142EFR
CSB-MP326142EFR
CSB-EP326142EFR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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