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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Crotalus scutulatus scutulatus Mojave toxin acidic chain CSB-YP325629CXM
CSB-EP325629CXM
CSB-BP325629CXM
CSB-MP325629CXM
CSB-EP325629CXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspidelaps scutatus Cytotoxin homolog S3C2 CSB-YP325630APV
CSB-BP325630APV
CSB-MP325630APV
CSB-EP325630APV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline immunodeficiency virus Probable protein Rev (rev) CSB-YP325631FAM
CSB-EP325631FAM
CSB-BP325631FAM
CSB-MP325631FAM
CSB-EP325631FAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE (nifE) CSB-YP325632RLC
CSB-EP325632RLC
CSB-BP325632RLC
CSB-MP325632RLC
CSB-EP325632RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Baseplate structural protein Gp6 (6), partial CSB-YP325633EDZ
CSB-EP325633EDZ
CSB-BP325633EDZ
CSB-MP325633EDZ
CSB-EP325633EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Nitrogenase molybdenum-iron protein alpha chain (nifD1), partial CSB-YP325634RKX
CSB-EP325634RKX
CSB-BP325634RKX
CSB-MP325634RKX
CSB-EP325634RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cell division control protein 42 (CDC42) CSB-YP325635SVG
CSB-EP325635SVG
CSB-BP325635SVG
CSB-MP325635SVG
CSB-EP325635SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Phosrestin-1 (Arr2) CSB-YP325636DLU
CSB-EP325636DLU
CSB-BP325636DLU
CSB-MP325636DLU
CSB-EP325636DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macropus giganteus Alpha-lactalbumin I (LALBA) CSB-YP325637MPF
CSB-EP325637MPF
CSB-BP325637MPF
CSB-MP325637MPF
CSB-EP325637MPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bean leafroll virus Movement protein (ORF4) CSB-YP325638BFT
CSB-EP325638BFT
CSB-BP325638BFT
CSB-MP325638BFT
CSB-EP325638BFT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae General amino-acid permease GAP1 (GAP1), partial CSB-YP325639SVG
CSB-EP325639SVG
CSB-BP325639SVG
CSB-MP325639SVG
CSB-EP325639SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine adenovirus 7 Adenain CSB-YP325640BJD
CSB-EP325640BJD
CSB-BP325640BJD
CSB-MP325640BJD
CSB-EP325640BJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP325641FBQ
CSB-EP325641FBQ
CSB-BP325641FBQ
CSB-MP325641FBQ
CSB-EP325641FBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP325642EHU
CSB-EP325642EHU
CSB-BP325642EHU
CSB-MP325642EHU
CSB-EP325642EHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Acidic endochitinase (CHIB1) CSB-YP325643DOA
CSB-EP325643DOA
CSB-BP325643DOA
CSB-MP325643DOA
CSB-EP325643DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petroselinum crispum Histone H2A CSB-YP325644PGL
CSB-EP325644PGL
CSB-BP325644PGL
CSB-MP325644PGL
CSB-EP325644PGL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus Ig heavy chain V region 5A CSB-YP325645DQG
CSB-EP325645DQG
CSB-BP325645DQG
CSB-MP325645DQG
CSB-EP325645DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia phage 1 DNA binding protein ORF8 (ORF8) CSB-YP325646CIV
CSB-EP325646CIV
CSB-BP325646CIV
CSB-MP325646CIV
CSB-EP325646CIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage BF23 Uncharacterized 9.2 kDa protein CSB-YP325647ECE
CSB-EP325647ECE
CSB-BP325647ECE
CSB-MP325647ECE
CSB-EP325647ECE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Commelina yellow mottle virus Uncharacterized 15 kDa protein CSB-YP325648CNO
CSB-EP325648CNO
CSB-BP325648CNO
CSB-MP325648CNO
CSB-EP325648CNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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