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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Respiratory nitrate reductase 1 alpha chain (narG), partial CSB-YP362605ENV
CSB-BP362605ENV
CSB-MP362605ENV
CSB-EP362605ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribonuclease D (rnd) CSB-YP362606ENV
CSB-EP362606ENV
CSB-BP362606ENV
CSB-MP362606ENV
CSB-EP362606ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yjaA (yjaA) CSB-YP362607ENV
CSB-EP362607ENV
CSB-BP362607ENV
CSB-MP362607ENV
CSB-EP362607ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized N-acetyltransferase YjaB (yjaB) CSB-YP362608ENV
CSB-EP362608ENV
CSB-BP362608ENV
CSB-MP362608ENV
CSB-EP362608ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila Aerolysin (aerA) CSB-YP362609AXW
CSB-EP362609AXW
CSB-BP362609AXW
CSB-MP362609AXW
CSB-EP362609AXW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine transmissible gastroenteritis coronavirus Membrane protein (M), partial CSB-YP362610PQF1
CSB-EP362610PQF1
CSB-BP362610PQF1
CSB-MP362610PQF1
CSB-EP362610PQF1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mucorpepsin CSB-YP362611RKZ
CSB-EP362611RKZ
CSB-BP362611RKZ
CSB-MP362611RKZ
CSB-EP362611RKZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana glauca Protein-tyrosine phosphatase rolB (ROLB) CSB-YP362612NDJ
CSB-EP362612NDJ
CSB-BP362612NDJ
CSB-MP362612NDJ
CSB-EP362612NDJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Colicin-N immunity protein (cni), partial CSB-YP362613ENL1
CSB-EP362613ENL1
CSB-BP362613ENL1
CSB-MP362613ENL1
CSB-EP362613ENL1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Seed linoleate 9S-lipoxygenase-3 (LOX1.3), partial CSB-YP362614GGV
CSB-EP362614GGV
CSB-BP362614GGV
CSB-MP362614GGV
CSB-EP362614GGV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petunia hybrida Heat shock cognate 70 kDa protein (HSP70), partial CSB-YP362615EUD
CSB-EP362615EUD
CSB-BP362615EUD
CSB-MP362615EUD
CSB-EP362615EUD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Photosystem II 44 kDa reaction center protein (psbC), partial CSB-YP362616SSQ1
CSB-EP362616SSQ1
CSB-BP362616SSQ1
CSB-MP362616SSQ1
CSB-EP362616SSQ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Tubulin alpha-2 chain (TUBA2) CSB-YP362617DSJ
CSB-EP362617DSJ
CSB-BP362617DSJ
CSB-MP362617DSJ
CSB-EP362617DSJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Superoxide dismutase [Fe] (sodB) CSB-YP362618FFZ
CSB-EP362618FFZ
CSB-BP362618FFZ
CSB-MP362618FFZ
CSB-EP362618FFZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 25 kDa calcium-binding protein CSB-YP362619TLU
CSB-EP362619TLU
CSB-BP362619TLU
CSB-MP362619TLU
CSB-EP362619TLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Alkaline extracellular protease (XPR2) CSB-YP362620YAP
CSB-EP362620YAP
CSB-BP362620YAP
CSB-MP362620YAP
CSB-EP362620YAP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cerevisin (PRB1), partial CSB-YP362621SVG
CSB-EP362621SVG
CSB-BP362621SVG
CSB-MP362621SVG
CSB-EP362621SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin 2 (groL2), partial CSB-YP362622MVN
CSB-EP362622MVN
CSB-BP362622MVN
CSB-MP362622MVN
CSB-EP362622MVN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin alpha-2 chain CSB-YP362623SQX
CSB-EP362623SQX
CSB-BP362623SQX
CSB-MP362623SQX
CSB-EP362623SQX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Major capsid protein (40), partial CSB-YP362624VAP
CSB-EP362624VAP
CSB-BP362624VAP
CSB-MP362624VAP
CSB-EP362624VAP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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