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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Apis mellifera Homeobox protein E60 CSB-YP362585DNK
CSB-EP362585DNK
CSB-BP362585DNK
CSB-MP362585DNK
CSB-EP362585DNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Homeotic protein Sex combs reduced (Scr) CSB-YP362586DLU
CSB-EP362586DLU
CSB-BP362586DLU
CSB-MP362586DLU
CSB-EP362586DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Homeotic protein bicoid (bcd) CSB-YP362587DLU
CSB-EP362587DLU
CSB-BP362587DLU
CSB-MP362587DLU
CSB-EP362587DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Homeotic protein caudal (cad) CSB-YP362588DLU
CSB-EP362588DLU
CSB-BP362588DLU
CSB-MP362588DLU
CSB-EP362588DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Homeobox protein abdominal-B (Abd-B) CSB-YP362589DLU
CSB-EP362589DLU
CSB-BP362589DLU
CSB-MP362589DLU
CSB-EP362589DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein zerknuellt 1 (zen) CSB-YP362590DLU
CSB-EP362590DLU
CSB-BP362590DLU
CSB-MP362590DLU
CSB-EP362590DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Glyceraldehyde-3-phosphate dehydrogenase, cytosolic (GAPC) CSB-YP362591NHE
CSB-EP362591NHE
CSB-BP362591NHE
CSB-MP362591NHE
CSB-EP362591NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Acetolactate synthase 2, chloroplastic (ALS SURB), partial CSB-YP362592NHE
CSB-EP362592NHE
CSB-BP362592NHE
CSB-MP362592NHE
CSB-EP362592NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cell division control protein 6 (CDC6), partial CSB-YP362593SVG
CSB-EP362593SVG
CSB-BP362593SVG
CSB-MP362593SVG
CSB-EP362593SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus sp. Cyclomaltodextrin glucanotransferase (cgt), partial CSB-YP362594BBS
CSB-EP362594BBS
CSB-BP362594BBS
CSB-MP362594BBS
CSB-EP362594BBS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus sp. Threonine synthase (thrC) CSB-YP362595BCK
CSB-EP362595BCK
CSB-BP362595BCK
CSB-MP362595BCK
CSB-EP362595BCK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eimeria acervulina Merozoite surface protein CMZ-8 CSB-YP362596EBA
CSB-EP362596EBA
CSB-BP362596EBA
CSB-MP362596EBA
CSB-EP362596EBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uroporphyrinogen-III C-methyltransferase (hemX), partial CSB-YP362597ENV1
CSB-EP362597ENV1
CSB-BP362597ENV1
CSB-MP362597ENV1
CSB-EP362597ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Coupling protein TraD (traD), partial CSB-YP362598ENV1
CSB-EP362598ENV1
CSB-BP362598ENV1
CSB-MP362598ENV1
CSB-EP362598ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein nifT (nifT) CSB-YP362599KBG
CSB-EP362599KBG
CSB-BP362599KBG
CSB-MP362599KBG
CSB-EP362599KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein nifX (nifX) CSB-YP362600KBG
CSB-EP362600KBG
CSB-BP362600KBG
CSB-MP362600KBG
CSB-EP362600KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkanal monooxygenase alpha chain (luxA) CSB-YP362601EUW
CSB-EP362601EUW
CSB-BP362601EUW
CSB-MP362601EUW
CSB-EP362601EUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Non-fluorescent flavoprotein (luxF) CSB-YP362602EUW
CSB-EP362602EUW
CSB-BP362602EUW
CSB-MP362602EUW
CSB-EP362602EUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Photosystem I P700 chlorophyll a apoprotein A2 (psaB), partial CSB-YP362603DSJ
CSB-EP362603DSJ
CSB-BP362603DSJ
CSB-MP362603DSJ
CSB-EP362603DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Outer membrane protein A (ompA), partial CSB-YP362604EKN
CSB-EP362604EKN
CSB-BP362604EKN
CSB-MP362604EKN
CSB-EP362604EKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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