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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Candida albicans Acetyl-CoA carboxylase (ACC1), partial CSB-EP2727CZD 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Human Valacyclovir hydrolase (BPHL) CSB-YP774821HU(F1)a1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (CD38), partial CSB-YP004929HU1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B), partial CSB-YP023978HU2 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Valacyclovir hydrolase (BPHL) CSB-EP774821HU(F1) 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Bacillus subtilis Subtilosin-A (sboA) CSB-EP522484BRJe3 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Peptidyl-prolyl cis-trans isomerase A (PPIA) CSB-EP351810HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Mouse Sushi domain-containing protein 4 (Susd4), partial CSB-EP805887MOa0 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Fibroblast growth factor 1 (FGF1) CSB-EP008615HUc7 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human ATP-binding cassette sub-family G member 1 (ABCG1), partial CSB-YP001080HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Isthmin-1 (ISM1) CSB-EP011850HUa0 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Mouse Prohibitin (Phb), partial CSB-EP017885MO1 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Human respiratory syncytial virus A Fusion glycoprotein F0 (F), partial CSB-EP356041HPOa0 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Variola virus A27L protein (A27L) CSB-EP2678VAR 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Oryza sativa subsp. japonica TPR repeat-containing thioredoxin TDX (Os09g0401200) CSB-EP732309OFGb1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Platelet-derived growth factor subunit B (PDGFB) CSB-EP017709HUc7 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Variola virus A27L protein (A27L) CSB-MP2678VAR 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Rat Glucagon-like peptide 1 receptor (Glp1r), partial CSB-YP009514RA 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Bovine Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (PPP2CA) CSB-YP018559BO
CSB-EP018559BO
CSB-BP018559BO
CSB-MP018559BO
CSB-EP018559BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073DO1
CSB-EP015073DO1
CSB-BP015073DO1
CSB-MP015073DO1
CSB-EP015073DO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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