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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanoculleus marisnigri O-phospho-L-seryl-tRNA:Cys-tRNA synthase (Memar_1614) CSB-YP383877MNU
CSB-EP383877MNU
CSB-BP383877MNU
CSB-MP383877MNU
CSB-EP383877MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 50S ribosomal protein L15e (rpl15e) CSB-YP383878MNU
CSB-EP383878MNU
CSB-BP383878MNU
CSB-MP383878MNU
CSB-EP383878MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Aspartate carbamoyltransferase (pyrB) CSB-YP383879MNU
CSB-EP383879MNU
CSB-BP383879MNU
CSB-MP383879MNU
CSB-EP383879MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri tRNA (guanine (26)-N (2))-dimethyltransferase CSB-YP383880MNU
CSB-EP383880MNU
CSB-BP383880MNU
CSB-MP383880MNU
CSB-EP383880MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 30S ribosomal protein S11P (rps11p) CSB-YP383881MNU
CSB-EP383881MNU
CSB-BP383881MNU
CSB-MP383881MNU
CSB-EP383881MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 2-phospho-L-lactate guanylyltransferase (cofC) CSB-YP383882MNU
CSB-EP383882MNU
CSB-BP383882MNU
CSB-MP383882MNU
CSB-EP383882MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Nitrogenase iron protein (nifH) CSB-YP383883MNU
CSB-EP383883MNU
CSB-BP383883MNU
CSB-MP383883MNU
CSB-EP383883MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 50S ribosomal protein L21e (rpl21e) CSB-YP383884MNU
CSB-EP383884MNU
CSB-BP383884MNU
CSB-MP383884MNU
CSB-EP383884MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Ribonuclease P protein component 4 (rnp4) CSB-YP383885MNU
CSB-EP383885MNU
CSB-BP383885MNU
CSB-MP383885MNU
CSB-EP383885MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri UPF0059 membrane protein Memar_2039 (Memar_2039), partial CSB-YP383886MNU1
CSB-EP383886MNU1
CSB-BP383886MNU1
CSB-MP383886MNU1
CSB-EP383886MNU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP383887MNU
CSB-EP383887MNU
CSB-BP383887MNU
CSB-MP383887MNU
CSB-EP383887MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri DNA repair and recombination protein radB (radB) CSB-YP383888MNU
CSB-EP383888MNU
CSB-BP383888MNU
CSB-MP383888MNU
CSB-EP383888MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Ketol-acid reductoisomerase (ilvC) CSB-YP383889MNU
CSB-EP383889MNU
CSB-BP383889MNU
CSB-MP383889MNU
CSB-EP383889MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri O-phosphoserine--tRNA (Cys) ligase, partial CSB-YP383890MNU
CSB-EP383890MNU
CSB-BP383890MNU
CSB-MP383890MNU
CSB-EP383890MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein (Memar_2247), partial CSB-YP383891MNU
CSB-EP383891MNU
CSB-BP383891MNU
CSB-MP383891MNU
CSB-EP383891MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP383892STL
CSB-EP383892STL
CSB-BP383892STL
CSB-MP383892STL
CSB-EP383892STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP383893STL
CSB-EP383893STL
CSB-BP383893STL
CSB-MP383893STL
CSB-EP383893STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP383894STL
CSB-EP383894STL
CSB-BP383894STL
CSB-MP383894STL
CSB-EP383894STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP383895STL
CSB-EP383895STL
CSB-BP383895STL
CSB-MP383895STL
CSB-EP383895STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0345 protein Sbal_0273 (Sbal_0273) CSB-YP383896STL
CSB-EP383896STL
CSB-BP383896STL
CSB-MP383896STL
CSB-EP383896STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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