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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Danio rerio Succinate dehydrogenase assembly factor 2, mitochondrial (sdhaf2) CSB-YP385278DIL
CSB-EP385278DIL
CSB-BP385278DIL
CSB-MP385278DIL
CSB-EP385278DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Small RNA degrading nuclease 1 (SDN1) CSB-YP385279DOA
CSB-EP385279DOA
CSB-BP385279DOA
CSB-MP385279DOA
CSB-EP385279DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g79080, chloroplastic (At1g79080), partial CSB-YP385280DOA
CSB-EP385280DOA
CSB-BP385280DOA
CSB-MP385280DOA
CSB-EP385280DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Large proline-rich protein BAG6 (Bag6), partial CSB-YP385281DIL
CSB-EP385281DIL
CSB-BP385281DIL
CSB-MP385281DIL
CSB-EP385281DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Outer dense fiber protein 3-B (odf3b) CSB-YP385282DIL
CSB-EP385282DIL
CSB-BP385282DIL
CSB-MP385282DIL
CSB-EP385282DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Golgi to ER traffic protein 1 (GET1), partial CSB-YP385283SXM1
CSB-EP385283SXM1
CSB-BP385283SXM1
CSB-MP385283SXM1
CSB-EP385283SXM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Mitochondrial genome required protein 1 (MGR1), partial CSB-YP385284SXM1
CSB-EP385284SXM1
CSB-BP385284SXM1
CSB-MP385284SXM1
CSB-EP385284SXM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis UPF0507 protein PICST_55861 (PICST_55861), partial CSB-YP385285SXM
CSB-EP385285SXM
CSB-BP385285SXM
CSB-MP385285SXM
CSB-EP385285SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 (PRP28) CSB-YP385286SXM
CSB-EP385286SXM
CSB-BP385286SXM
CSB-MP385286SXM
CSB-EP385286SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Regulator of rDNA transcription 14 (RRT14) CSB-YP385287SXM
CSB-EP385287SXM
CSB-BP385287SXM
CSB-MP385287SXM
CSB-EP385287SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis ATP-dependent RNA helicase DBP8 (DBP8) CSB-YP385288SXM
CSB-EP385288SXM
CSB-BP385288SXM
CSB-MP385288SXM
CSB-EP385288SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis tRNA (guanine-N (7)-)-methyltransferase subunit TRM82 CSB-YP385289SXM
CSB-EP385289SXM
CSB-BP385289SXM
CSB-MP385289SXM
CSB-EP385289SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis SAGA-associated factor 11 (SGF11) CSB-YP385290SXM
CSB-EP385290SXM
CSB-BP385290SXM
CSB-MP385290SXM
CSB-EP385290SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis ATP-dependent RNA helicase DED1 (DED1), partial CSB-YP385291SXM
CSB-EP385291SXM
CSB-BP385291SXM
CSB-MP385291SXM
CSB-EP385291SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Protein DML1 (DML1) CSB-YP385292SXM
CSB-EP385292SXM
CSB-BP385292SXM
CSB-MP385292SXM
CSB-EP385292SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Cap-associated protein CAF20 (CAF20) CSB-YP385293SXM
CSB-EP385293SXM
CSB-BP385293SXM
CSB-MP385293SXM
CSB-EP385293SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Adenylyltransferase and sulfurtransferase UBA4 (UBA4) CSB-YP385294SXM
CSB-EP385294SXM
CSB-BP385294SXM
CSB-MP385294SXM
CSB-EP385294SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Protein FMP52-3, mitochondrial (FMP523) CSB-YP385295SXM
CSB-EP385295SXM
CSB-BP385295SXM
CSB-MP385295SXM
CSB-EP385295SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Formation of crista junctions protein 1 (FCJ1), partial CSB-YP385296SXM1
CSB-EP385296SXM1
CSB-BP385296SXM1
CSB-MP385296SXM1
CSB-EP385296SXM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis ATP-dependent RNA helicase DBP2 (DBP2), partial CSB-YP385297SXM
CSB-EP385297SXM
CSB-BP385297SXM
CSB-MP385297SXM
CSB-EP385297SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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