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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia pseudomallei Urease accessory protein UreE (ureE) CSB-YP384477BPU
CSB-EP384477BPU
CSB-BP384477BPU
CSB-MP384477BPU
CSB-EP384477BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Aspartate carbamoyltransferase (pyrB) CSB-YP384478BPU
CSB-EP384478BPU
CSB-BP384478BPU
CSB-MP384478BPU
CSB-EP384478BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UPF0226 membrane protein BURPS668_3162 (BURPS668_3162), partial CSB-YP384479BPU
CSB-EP384479BPU
CSB-BP384479BPU
CSB-MP384479BPU
CSB-EP384479BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP384480BPU
CSB-EP384480BPU
CSB-BP384480BPU
CSB-MP384480BPU
CSB-EP384480BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP384481BPU1
CSB-EP384481BPU1
CSB-BP384481BPU1
CSB-MP384481BPU1
CSB-EP384481BPU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S9 (rpsI) CSB-YP384482BPU
CSB-EP384482BPU
CSB-BP384482BPU
CSB-MP384482BPU
CSB-EP384482BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Ribosomal protein L11 methyltransferase (prmA) CSB-YP384483BPU
CSB-EP384483BPU
CSB-BP384483BPU
CSB-MP384483BPU
CSB-EP384483BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei GTPase obg (obg) CSB-YP384484BPU
CSB-EP384484BPU
CSB-BP384484BPU
CSB-MP384484BPU
CSB-EP384484BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UPF0289 protein BURPS668_3510 (BURPS668_3510) CSB-YP384485BPU
CSB-EP384485BPU
CSB-BP384485BPU
CSB-MP384485BPU
CSB-EP384485BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei D-alanine--D-alanine ligase (ddl) CSB-YP384486BPU
CSB-EP384486BPU
CSB-BP384486BPU
CSB-MP384486BPU
CSB-EP384486BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD), partial CSB-YP384487BPU
CSB-EP384487BPU
CSB-BP384487BPU
CSB-MP384487BPU
CSB-EP384487BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP384488BPU
CSB-EP384488BPU
CSB-BP384488BPU
CSB-MP384488BPU
CSB-EP384488BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Anthranilate phosphoribosyltransferase (trpD) CSB-YP384489BPU
CSB-EP384489BPU
CSB-BP384489BPU
CSB-MP384489BPU
CSB-EP384489BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Glutamyl-tRNA reductase (hemA) CSB-YP384490BPU
CSB-EP384490BPU
CSB-BP384490BPU
CSB-MP384490BPU
CSB-EP384490BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP384491BPU
CSB-EP384491BPU
CSB-BP384491BPU
CSB-MP384491BPU
CSB-EP384491BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S4 (rpsD) CSB-YP384492BPU
CSB-EP384492BPU
CSB-BP384492BPU
CSB-MP384492BPU
CSB-EP384492BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Translation initiation factor IF-1 2 (infA2) CSB-YP384493BPU
CSB-EP384493BPU
CSB-BP384493BPU
CSB-MP384493BPU
CSB-EP384493BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L6 (rplF) CSB-YP384494BPU
CSB-EP384494BPU
CSB-BP384494BPU
CSB-MP384494BPU
CSB-EP384494BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S14 (rpsN) CSB-YP384495BPU
CSB-EP384495BPU
CSB-BP384495BPU
CSB-MP384495BPU
CSB-EP384495BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S17 (rpsQ) CSB-YP384496BPU
CSB-EP384496BPU
CSB-BP384496BPU
CSB-MP384496BPU
CSB-EP384496BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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