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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharopolyspora erythraea Bifunctional protein FolD 2 (folD2) CSB-YP389222STB
CSB-EP389222STB
CSB-BP389222STB
CSB-MP389222STB
CSB-EP389222STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L13 (rplM) CSB-YP389223STB
CSB-EP389223STB
CSB-BP389223STB
CSB-MP389223STB
CSB-EP389223STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 30S ribosomal protein S11 (rpsK) CSB-YP389224STB
CSB-EP389224STB
CSB-BP389224STB
CSB-MP389224STB
CSB-EP389224STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L36 2 (rpmJ2) CSB-YP389225STB
CSB-EP389225STB
CSB-BP389225STB
CSB-MP389225STB
CSB-EP389225STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L24 (rplX) CSB-YP389226STB
CSB-EP389226STB
CSB-BP389226STB
CSB-MP389226STB
CSB-EP389226STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L16 (rplP) CSB-YP389227STB
CSB-EP389227STB
CSB-BP389227STB
CSB-MP389227STB
CSB-EP389227STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L22 (rplV) CSB-YP389228STB
CSB-EP389228STB
CSB-BP389228STB
CSB-MP389228STB
CSB-EP389228STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L3 (rplC) CSB-YP389229STB
CSB-EP389229STB
CSB-BP389229STB
CSB-MP389229STB
CSB-EP389229STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP389230STB
CSB-EP389230STB
CSB-BP389230STB
CSB-MP389230STB
CSB-EP389230STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea UPF0336 protein SACE_6876 (SACE_6876) CSB-YP389231STB
CSB-EP389231STB
CSB-BP389231STB
CSB-MP389231STB
CSB-EP389231STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea UPF0234 protein SACE_6882 (SACE_6882) CSB-YP389232STB
CSB-EP389232STB
CSB-BP389232STB
CSB-MP389232STB
CSB-EP389232STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP389233STB
CSB-EP389233STB
CSB-BP389233STB
CSB-MP389233STB
CSB-EP389233STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP389234STB
CSB-EP389234STB
CSB-BP389234STB
CSB-MP389234STB
CSB-EP389234STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Glutamyl-tRNA reductase (hemA) CSB-YP389235STB
CSB-EP389235STB
CSB-BP389235STB
CSB-MP389235STB
CSB-EP389235STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Mycothiol acetyltransferase (mshD) CSB-YP389236STB
CSB-EP389236STB
CSB-BP389236STB
CSB-MP389236STB
CSB-EP389236STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 30S ribosomal protein S18 2 (rpsR2) CSB-YP389237STB
CSB-EP389237STB
CSB-BP389237STB
CSB-MP389237STB
CSB-EP389237STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 30S ribosomal protein S6 (rpsF) CSB-YP389238STB
CSB-EP389238STB
CSB-BP389238STB
CSB-MP389238STB
CSB-EP389238STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cutaneous T-cell lymphoma-associated antigen 9 (CTAGE9), partial CSB-YP389239HU1
CSB-EP389239HU1
CSB-BP389239HU1
CSB-MP389239HU1
CSB-EP389239HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Putative oxidoreductase GLYR1 (GLYR1), partial CSB-YP389240BO
CSB-EP389240BO
CSB-BP389240BO
CSB-MP389240BO
CSB-EP389240BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Septin-8-B (sept8b) CSB-YP389241DIL
CSB-EP389241DIL
CSB-BP389241DIL
CSB-MP389241DIL
CSB-EP389241DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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