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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus maripaludis Biotin synthase (bioB) CSB-YP389262MNP
CSB-EP389262MNP
CSB-BP389262MNP
CSB-MP389262MNP
CSB-EP389262MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable cobalt-precorrin-6Y C (15)-methyltransferase [decarboxylating] CSB-YP389263MNP
CSB-EP389263MNP
CSB-BP389263MNP
CSB-MP389263MNP
CSB-EP389263MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable cobyric acid synthase (cobQ) CSB-YP389264MNP
CSB-EP389264MNP
CSB-BP389264MNP
CSB-MP389264MNP
CSB-EP389264MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S6e (rps6e) CSB-YP389265MNP
CSB-EP389265MNP
CSB-BP389265MNP
CSB-MP389265MNP
CSB-EP389265MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP389266MNP
CSB-EP389266MNP
CSB-BP389266MNP
CSB-MP389266MNP
CSB-EP389266MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable peroxiredoxin (MmarC5_0413) CSB-YP389267MNP
CSB-EP389267MNP
CSB-BP389267MNP
CSB-MP389267MNP
CSB-EP389267MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Thiamine-phosphate synthase (thiE) CSB-YP389268MNP
CSB-EP389268MNP
CSB-BP389268MNP
CSB-MP389268MNP
CSB-EP389268MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP389269MNP
CSB-EP389269MNP
CSB-BP389269MNP
CSB-MP389269MNP
CSB-EP389269MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Bifunctional protein GlmU (MmarC5_0513) CSB-YP389270MNP
CSB-EP389270MNP
CSB-BP389270MNP
CSB-MP389270MNP
CSB-EP389270MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis V-type ATP synthase subunit D (atpD) CSB-YP389271MNP
CSB-EP389271MNP
CSB-BP389271MNP
CSB-MP389271MNP
CSB-EP389271MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis V-type ATP synthase subunit E (atpE) CSB-YP389272MNP
CSB-EP389272MNP
CSB-BP389272MNP
CSB-MP389272MNP
CSB-EP389272MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Anthranilate phosphoribosyltransferase (trpD) CSB-YP389273MNP
CSB-EP389273MNP
CSB-BP389273MNP
CSB-MP389273MNP
CSB-EP389273MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis FO synthase subunit 1 (cofG) CSB-YP389274MNP
CSB-EP389274MNP
CSB-BP389274MNP
CSB-MP389274MNP
CSB-EP389274MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribonuclease Z (rnz) CSB-YP389275MNP
CSB-EP389275MNP
CSB-BP389275MNP
CSB-MP389275MNP
CSB-EP389275MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Urease accessory protein UreD 2 (ureD2) CSB-YP389276STB
CSB-EP389276STB
CSB-BP389276STB
CSB-MP389276STB
CSB-EP389276STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Inositol 2-dehydrogenase 1 (iolG1) CSB-YP389277STB
CSB-EP389277STB
CSB-BP389277STB
CSB-MP389277STB
CSB-EP389277STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Undecaprenyl-diphosphatase (uppP), partial CSB-YP389278STB1
CSB-EP389278STB1
CSB-BP389278STB1
CSB-MP389278STB1
CSB-EP389278STB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Bifunctional protein FolD 1 (folD1) CSB-YP389279STB
CSB-EP389279STB
CSB-BP389279STB
CSB-MP389279STB
CSB-EP389279STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Catalase-peroxidase (katG), partial CSB-YP389280STB
CSB-EP389280STB
CSB-BP389280STB
CSB-MP389280STB
CSB-EP389280STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Isopentenyl-diphosphate Delta-isomerase (idi) CSB-YP389281STB
CSB-EP389281STB
CSB-BP389281STB
CSB-MP389281STB
CSB-EP389281STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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