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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Phaseolus vulgaris Photosystem I assembly protein ycf3 (ycf3) CSB-YP390062EUN
CSB-EP390062EUN
CSB-BP390062EUN
CSB-MP390062EUN
CSB-EP390062EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP390063EUN1
CSB-EP390063EUN1
CSB-BP390063EUN1
CSB-MP390063EUN1
CSB-EP390063EUN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP390064EUN
CSB-EP390064EUN
CSB-BP390064EUN
CSB-MP390064EUN
CSB-EP390064EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP390065EUN
CSB-EP390065EUN
CSB-BP390065EUN
CSB-MP390065EUN
CSB-EP390065EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP390066EUN1
CSB-EP390066EUN1
CSB-BP390066EUN1
CSB-MP390066EUN1
CSB-EP390066EUN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Photosystem II reaction center protein J (psbJ), partial CSB-YP390067EUN
CSB-EP390067EUN
CSB-BP390067EUN
CSB-MP390067EUN
CSB-EP390067EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Photosystem I reaction center subunit IX (psaJ), partial CSB-YP390068EUN
CSB-EP390068EUN
CSB-BP390068EUN
CSB-MP390068EUN
CSB-EP390068EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP390069EUN
CSB-EP390069EUN
CSB-BP390069EUN
CSB-MP390069EUN
CSB-EP390069EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP390070EUN
CSB-EP390070EUN
CSB-BP390070EUN
CSB-MP390070EUN
CSB-EP390070EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Protein ycf2 (ycf2-A), partial CSB-YP390071EUN
CSB-EP390071EUN
CSB-BP390071EUN
CSB-MP390071EUN
CSB-EP390071EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris NAD (P)H-quinone oxidoreductase chain 4, chloroplastic, partial CSB-YP390072EUN
CSB-EP390072EUN
CSB-BP390072EUN
CSB-MP390072EUN
CSB-EP390072EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris NAD (P)H-quinone oxidoreductase subunit 4L, chloroplastic, partial CSB-YP390073EUN1
CSB-EP390073EUN1
CSB-BP390073EUN1
CSB-MP390073EUN1
CSB-EP390073EUN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Putative membrane protein ycf1 (ycf1), partial CSB-YP390074EUN
CSB-EP390074EUN
CSB-BP390074EUN
CSB-MP390074EUN
CSB-EP390074EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris 50S ribosomal protein L2, chloroplastic (rpl2-A) CSB-YP390075EUN
CSB-EP390075EUN
CSB-BP390075EUN
CSB-MP390075EUN
CSB-EP390075EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa Maturase K (matK), partial CSB-YP390076EXS
CSB-EP390076EXS
CSB-BP390076EXS
CSB-MP390076EXS
CSB-EP390076EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP390077EXS1
CSB-EP390077EXS1
CSB-BP390077EXS1
CSB-MP390077EXS1
CSB-EP390077EXS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa 30S ribosomal protein S2, chloroplastic (rps2) CSB-YP390078EXS
CSB-EP390078EXS
CSB-BP390078EXS
CSB-MP390078EXS
CSB-EP390078EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP390079EXS
CSB-EP390079EXS
CSB-BP390079EXS
CSB-MP390079EXS
CSB-EP390079EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa 30S ribosomal protein S14, chloroplastic (rps14) CSB-YP390080EXS
CSB-EP390080EXS
CSB-BP390080EXS
CSB-MP390080EXS
CSB-EP390080EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa Photosystem I P700 chlorophyll a apoprotein A1 (psaA), partial CSB-YP390081EXS
CSB-EP390081EXS
CSB-BP390081EXS
CSB-MP390081EXS
CSB-EP390081EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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