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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacillus thermodenitrificans Enolase (eno) CSB-YP390202GEH
CSB-EP390202GEH
CSB-BP390202GEH
CSB-MP390202GEH
CSB-EP390202GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP390203GEH
CSB-EP390203GEH
CSB-BP390203GEH
CSB-MP390203GEH
CSB-EP390203GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans ATP phosphoribosyltransferase (hisG) CSB-YP390204GEH
CSB-EP390204GEH
CSB-BP390204GEH
CSB-MP390204GEH
CSB-EP390204GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Carbon storage regulator homolog (csrA) CSB-YP390205GEH
CSB-EP390205GEH
CSB-BP390205GEH
CSB-MP390205GEH
CSB-EP390205GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP390206GEH
CSB-EP390206GEH
CSB-BP390206GEH
CSB-MP390206GEH
CSB-EP390206GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP390207GEH
CSB-EP390207GEH
CSB-BP390207GEH
CSB-MP390207GEH
CSB-EP390207GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0059 membrane protein GTNG_3319 (GTNG_3319), partial CSB-YP390208GEH1
CSB-EP390208GEH1
CSB-BP390208GEH1
CSB-MP390208GEH1
CSB-EP390208GEH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0447 protein GTNG_3362 (GTNG_3362) CSB-YP390209GEH
CSB-EP390209GEH
CSB-BP390209GEH
CSB-MP390209GEH
CSB-EP390209GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Glycine--tRNA ligase (glyQS) CSB-YP390210GEH
CSB-EP390210GEH
CSB-BP390210GEH
CSB-MP390210GEH
CSB-EP390210GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP390211GEH
CSB-EP390211GEH
CSB-BP390211GEH
CSB-MP390211GEH
CSB-EP390211GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans tRNA modification GTPase MnmE (mnmE) CSB-YP390212GEH
CSB-EP390212GEH
CSB-BP390212GEH
CSB-MP390212GEH
CSB-EP390212GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 50S ribosomal protein L34 (rpmH) CSB-YP390213GEH
CSB-EP390213GEH
CSB-BP390213GEH
CSB-MP390213GEH
CSB-EP390213GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Chromosomal replication initiator protein DnaA (dnaA) CSB-YP390214FDX
CSB-EP390214FDX
CSB-BP390214FDX
CSB-MP390214FDX
CSB-EP390214FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Tryptophan synthase alpha chain (trpA) CSB-YP390215FDX
CSB-EP390215FDX
CSB-BP390215FDX
CSB-MP390215FDX
CSB-EP390215FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Potassium-transporting ATPase A chain (kdpA), partial CSB-YP390216FDX
CSB-EP390216FDX
CSB-BP390216FDX
CSB-MP390216FDX
CSB-EP390216FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP390217FDX
CSB-EP390217FDX
CSB-BP390217FDX
CSB-MP390217FDX
CSB-EP390217FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP390218FDX
CSB-EP390218FDX
CSB-BP390218FDX
CSB-MP390218FDX
CSB-EP390218FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Ribosome maturation factor RimP (rimP) CSB-YP390219FDX
CSB-EP390219FDX
CSB-BP390219FDX
CSB-MP390219FDX
CSB-EP390219FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP390220FDX
CSB-EP390220FDX
CSB-BP390220FDX
CSB-MP390220FDX
CSB-EP390220FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis 50S ribosomal protein L1 (rplA) CSB-YP390221FDX
CSB-EP390221FDX
CSB-BP390221FDX
CSB-MP390221FDX
CSB-EP390221FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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