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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Corynebacterium glutamicum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP391402CXG
CSB-EP391402CXG
CSB-BP391402CXG
CSB-MP391402CXG
CSB-EP391402CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Dihydroxy-acid dehydratase (ilvD), partial CSB-YP391403CXG
CSB-EP391403CXG
CSB-BP391403CXG
CSB-MP391403CXG
CSB-EP391403CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 3-isopropylmalate dehydrogenase (leuB) CSB-YP391404CXG
CSB-EP391404CXG
CSB-BP391404CXG
CSB-MP391404CXG
CSB-EP391404CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP391405CXG
CSB-EP391405CXG
CSB-BP391405CXG
CSB-MP391405CXG
CSB-EP391405CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L35 (rpmI) CSB-YP391406CXG
CSB-EP391406CXG
CSB-BP391406CXG
CSB-MP391406CXG
CSB-EP391406CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Arginine repressor (argR) CSB-YP391407CXG
CSB-EP391407CXG
CSB-BP391407CXG
CSB-MP391407CXG
CSB-EP391407CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Prokaryotic ubiquitin-like protein Pup (pup) CSB-YP391408CXG
CSB-EP391408CXG
CSB-BP391408CXG
CSB-MP391408CXG
CSB-EP391408CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum ATP phosphoribosyltransferase (hisG) CSB-YP391409CXG
CSB-EP391409CXG
CSB-BP391409CXG
CSB-MP391409CXG
CSB-EP391409CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Dihydroorotate dehydrogenase (quinone) CSB-YP391410CXG
CSB-EP391410CXG
CSB-BP391410CXG
CSB-MP391410CXG
CSB-EP391410CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum UvrABC system protein C (uvrC), partial CSB-YP391411CXG
CSB-EP391411CXG
CSB-BP391411CXG
CSB-MP391411CXG
CSB-EP391411CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP391412CXG
CSB-EP391412CXG
CSB-BP391412CXG
CSB-MP391412CXG
CSB-EP391412CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Elongation factor P (efp) CSB-YP391413CXG
CSB-EP391413CXG
CSB-BP391413CXG
CSB-MP391413CXG
CSB-EP391413CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP391414CXG
CSB-EP391414CXG
CSB-BP391414CXG
CSB-MP391414CXG
CSB-EP391414CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum D-tyrosyl-tRNA (Tyr) deacylase CSB-YP391415CXG
CSB-EP391415CXG
CSB-BP391415CXG
CSB-MP391415CXG
CSB-EP391415CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Diaminopimelate epimerase (dapF) CSB-YP391416CXG
CSB-EP391416CXG
CSB-BP391416CXG
CSB-MP391416CXG
CSB-EP391416CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Protein RecA (recA) CSB-YP391417CXG
CSB-EP391417CXG
CSB-BP391417CXG
CSB-MP391417CXG
CSB-EP391417CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum UPF0246 protein cgR_1824 (cgR_1824) CSB-YP391418CXG
CSB-EP391418CXG
CSB-BP391418CXG
CSB-MP391418CXG
CSB-EP391418CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum UPF0061 protein cgR_1828 (cgR_1828) CSB-YP391419CXG
CSB-EP391419CXG
CSB-BP391419CXG
CSB-MP391419CXG
CSB-EP391419CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum UPF0102 protein cgR_1859 (cgR_1859) CSB-YP391420CXG
CSB-EP391420CXG
CSB-BP391420CXG
CSB-MP391420CXG
CSB-EP391420CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Ribonuclease HII (rnhB) CSB-YP391421CXG
CSB-EP391421CXG
CSB-BP391421CXG
CSB-MP391421CXG
CSB-EP391421CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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