重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實驗室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項目中生成的蛋白質(zhì)。它們在許多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來支持您的研究,您來對地方了。
經(jīng)過16多年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達技術(shù),以及膜蛋白表達技術(shù)等。
產(chǎn)品特點
- 超過10,000種重組蛋白覆蓋大多數(shù)基因/靶點;
- 5種表達系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細(xì)胞、哺乳動物細(xì)胞和體外大腸桿菌。而體外大腸桿菌表達系統(tǒng)是華美生物的獨特表達系統(tǒng)。與傳統(tǒng)蛋白表達系統(tǒng)相比,體外大腸桿菌省略了許多過程,如質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標(biāo)簽,包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用,包括抗原、細(xì)胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細(xì)胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標(biāo)準(zhǔn)及其原料、體內(nèi)研究、質(zhì)譜標(biāo)準(zhǔn)、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動物副產(chǎn)品的動物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
---|---|---|
Recombinant Aeromonas salmonicida 50S ribosomal protein L6 (rplF) | CSB-YP390862AUI CSB-EP390862AUI CSB-BP390862AUI CSB-MP390862AUI CSB-EP390862AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida 50S ribosomal protein L24 (rplX) | CSB-YP390863AUI CSB-EP390863AUI CSB-BP390863AUI CSB-MP390863AUI CSB-EP390863AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida 50S ribosomal protein L2 (rplB) | CSB-YP390864AUI CSB-EP390864AUI CSB-BP390864AUI CSB-MP390864AUI CSB-EP390864AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida Pimelyl-[acyl-carrier protein] methyl ester esterase (bioH) | CSB-YP390865AUI CSB-EP390865AUI CSB-BP390865AUI CSB-MP390865AUI CSB-EP390865AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida Protein-export protein secB (secB) | CSB-YP390866AUI CSB-EP390866AUI CSB-BP390866AUI CSB-MP390866AUI CSB-EP390866AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida Coproporphyrinogen-III oxidase, aerobic (hemF) | CSB-YP390867AUI CSB-EP390867AUI CSB-BP390867AUI CSB-MP390867AUI CSB-EP390867AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida LexA repressor (lexA) | CSB-YP390868AUI CSB-EP390868AUI CSB-BP390868AUI CSB-MP390868AUI CSB-EP390868AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida 50S ribosomal protein L33 (rpmG) | CSB-YP390869AUI CSB-EP390869AUI CSB-BP390869AUI CSB-MP390869AUI CSB-EP390869AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) | CSB-YP390870AUI CSB-EP390870AUI CSB-BP390870AUI CSB-MP390870AUI CSB-EP390870AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida Glycine--tRNA ligase alpha subunit (glyQ) | CSB-YP390871AUI CSB-EP390871AUI CSB-BP390871AUI CSB-MP390871AUI CSB-EP390871AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida Anaerobic nitric oxide reductase flavorubredoxin (norV), partial | CSB-YP390872AUI CSB-EP390872AUI CSB-BP390872AUI CSB-MP390872AUI CSB-EP390872AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) | CSB-YP390873AUI CSB-EP390873AUI CSB-BP390873AUI CSB-MP390873AUI CSB-EP390873AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida Sec-independent protein translocase protein TatB (tatB), partial | CSB-YP390874AUI CSB-EP390874AUI CSB-BP390874AUI CSB-MP390874AUI CSB-EP390874AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida Protein CrcB homolog (crcB), partial | CSB-YP390875AUI1 CSB-EP390875AUI1 CSB-BP390875AUI1 CSB-MP390875AUI1 CSB-EP390875AUI1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida tRNA modification GTPase MnmE (mnmE) | CSB-YP390876AUI CSB-EP390876AUI CSB-BP390876AUI CSB-MP390876AUI CSB-EP390876AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida Ribonuclease P protein component (rnpA) | CSB-YP390877AUI CSB-EP390877AUI CSB-BP390877AUI CSB-MP390877AUI CSB-EP390877AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. 50S ribosomal protein L1 (rplA) | CSB-YP390878PYU CSB-EP390878PYU CSB-BP390878PYU CSB-MP390878PYU CSB-EP390878PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. DNA-directed RNA polymerase subunit beta (rpoB), partial | CSB-YP390879PYU CSB-EP390879PYU CSB-BP390879PYU CSB-MP390879PYU CSB-EP390879PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. 30S ribosomal protein S7 (rpsG) | CSB-YP390880PYU CSB-EP390880PYU CSB-BP390880PYU CSB-MP390880PYU CSB-EP390880PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. 30S ribosomal protein S10 (rpsJ) | CSB-YP390881PYU CSB-EP390881PYU CSB-BP390881PYU CSB-MP390881PYU CSB-EP390881PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達載體?
表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022