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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Corynebacterium glutamicum 30S ribosomal protein S12 (rpsL) CSB-YP391382CXG
CSB-EP391382CXG
CSB-BP391382CXG
CSB-MP391382CXG
CSB-EP391382CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L3 (rplC) CSB-YP391383CXG
CSB-EP391383CXG
CSB-BP391383CXG
CSB-MP391383CXG
CSB-EP391383CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 30S ribosomal protein S3 (rpsC) CSB-YP391384CXG
CSB-EP391384CXG
CSB-BP391384CXG
CSB-MP391384CXG
CSB-EP391384CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 30S ribosomal protein S8 (rpsH) CSB-YP391385CXG
CSB-EP391385CXG
CSB-BP391385CXG
CSB-MP391385CXG
CSB-EP391385CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L30 (rpmD) CSB-YP391386CXG
CSB-EP391386CXG
CSB-BP391386CXG
CSB-MP391386CXG
CSB-EP391386CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 30S ribosomal protein S4 (rpsD) CSB-YP391387CXG
CSB-EP391387CXG
CSB-BP391387CXG
CSB-MP391387CXG
CSB-EP391387CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Alanine racemase (alr) CSB-YP391388CXG
CSB-EP391388CXG
CSB-BP391388CXG
CSB-MP391388CXG
CSB-EP391388CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 10 kDa chaperonin (groS) CSB-YP391389CXG
CSB-EP391389CXG
CSB-BP391389CXG
CSB-MP391389CXG
CSB-EP391389CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Maf-like protein cgR_0825 (cgR_0825) CSB-YP391390CXG
CSB-EP391390CXG
CSB-BP391390CXG
CSB-MP391390CXG
CSB-EP391390CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Protein translocase subunit SecA 1 (secA1), partial CSB-YP391391CXG
CSB-EP391391CXG
CSB-BP391391CXG
CSB-MP391391CXG
CSB-EP391391CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Peptide chain release factor 2 (prfB) CSB-YP391392CXG
CSB-EP391392CXG
CSB-BP391392CXG
CSB-MP391392CXG
CSB-EP391392CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP391393CXG
CSB-EP391393CXG
CSB-BP391393CXG
CSB-MP391393CXG
CSB-EP391393CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L33 (rpmG) CSB-YP391394CXG
CSB-EP391394CXG
CSB-BP391394CXG
CSB-MP391394CXG
CSB-EP391394CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L31 type B (rpmE2) CSB-YP391395CXG
CSB-EP391395CXG
CSB-BP391395CXG
CSB-MP391395CXG
CSB-EP391395CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP391396CXG
CSB-EP391396CXG
CSB-BP391396CXG
CSB-MP391396CXG
CSB-EP391396CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Enolase (eno) CSB-YP391397CXG
CSB-EP391397CXG
CSB-BP391397CXG
CSB-MP391397CXG
CSB-EP391397CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Uncharacterized peptidase cgR_1176 (cgR_1176) CSB-YP391398CXG
CSB-EP391398CXG
CSB-BP391398CXG
CSB-MP391398CXG
CSB-EP391398CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Arginine--tRNA ligase (argS), partial CSB-YP391399CXG
CSB-EP391399CXG
CSB-BP391399CXG
CSB-MP391399CXG
CSB-EP391399CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum ATP synthase epsilon chain (atpC) CSB-YP391400CXG
CSB-EP391400CXG
CSB-BP391400CXG
CSB-MP391400CXG
CSB-EP391400CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum DNA ligase (ligA), partial CSB-YP391401CXG
CSB-EP391401CXG
CSB-BP391401CXG
CSB-MP391401CXG
CSB-EP391401CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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