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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas stutzeri tRNA (guanine-N (1)-)-methyltransferase CSB-YP395224PZP
CSB-EP395224PZP
CSB-BP395224PZP
CSB-MP395224PZP
CSB-EP395224PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri tRNA (mo5U34)-methyltransferase CSB-YP395225PZP
CSB-EP395225PZP
CSB-BP395225PZP
CSB-MP395225PZP
CSB-EP395225PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri UPF0745 protein PST_1364 (PST_1364) CSB-YP395226PZP
CSB-EP395226PZP
CSB-BP395226PZP
CSB-MP395226PZP
CSB-EP395226PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Probable M18 family aminopeptidase 2 (apeB) CSB-YP395227PZP
CSB-EP395227PZP
CSB-BP395227PZP
CSB-MP395227PZP
CSB-EP395227PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri UPF0270 protein PST_1436 (PST_1436) CSB-YP395228PZP
CSB-EP395228PZP
CSB-BP395228PZP
CSB-MP395228PZP
CSB-EP395228PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Ribonuclease HII (rnhB) CSB-YP395229PZP
CSB-EP395229PZP
CSB-BP395229PZP
CSB-MP395229PZP
CSB-EP395229PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP395230PZP
CSB-EP395230PZP
CSB-BP395230PZP
CSB-MP395230PZP
CSB-EP395230PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP395231PZP
CSB-EP395231PZP
CSB-BP395231PZP
CSB-MP395231PZP
CSB-EP395231PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP395232PZP
CSB-EP395232PZP
CSB-BP395232PZP
CSB-MP395232PZP
CSB-EP395232PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Coenzyme PQQ synthesis protein D (pqqD) CSB-YP395233PZP
CSB-EP395233PZP
CSB-BP395233PZP
CSB-MP395233PZP
CSB-EP395233PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Siroheme synthase (cysG) CSB-YP395234PZP
CSB-EP395234PZP
CSB-BP395234PZP
CSB-MP395234PZP
CSB-EP395234PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Translation initiation factor IF-1 (infA) CSB-YP395235PZP
CSB-EP395235PZP
CSB-BP395235PZP
CSB-MP395235PZP
CSB-EP395235PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Chorismate synthase (aroC) CSB-YP395236PZP
CSB-EP395236PZP
CSB-BP395236PZP
CSB-MP395236PZP
CSB-EP395236PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Na (+)-translocating NADH-quinone reductase subunit E, partial CSB-YP395237PZP1
CSB-EP395237PZP1
CSB-BP395237PZP1
CSB-MP395237PZP1
CSB-EP395237PZP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP395238PZP
CSB-EP395238PZP
CSB-BP395238PZP
CSB-MP395238PZP
CSB-EP395238PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Tryptophan synthase alpha chain (trpA) CSB-YP395239PZP
CSB-EP395239PZP
CSB-BP395239PZP
CSB-MP395239PZP
CSB-EP395239PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri GTPase Der (der) CSB-YP395240PZP
CSB-EP395240PZP
CSB-BP395240PZP
CSB-MP395240PZP
CSB-EP395240PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 60 kDa chaperonin (groL), partial CSB-YP395241PZP
CSB-EP395241PZP
CSB-BP395241PZP
CSB-MP395241PZP
CSB-EP395241PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Uracil phosphoribosyltransferase (upp) CSB-YP395242PZP
CSB-EP395242PZP
CSB-BP395242PZP
CSB-MP395242PZP
CSB-EP395242PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 3-dehydroquinate dehydratase (aroQ) CSB-YP395243PZP
CSB-EP395243PZP
CSB-BP395243PZP
CSB-MP395243PZP
CSB-EP395243PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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