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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Enterobacter sp. UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) CSB-YP396024EIZ
CSB-EP396024EIZ
CSB-BP396024EIZ
CSB-MP396024EIZ
CSB-EP396024EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0263 protein Ent638_2296 (Ent638_2296) CSB-YP396025EIZ
CSB-EP396025EIZ
CSB-BP396025EIZ
CSB-MP396025EIZ
CSB-EP396025EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Cell volume regulation protein A (cvrA), partial CSB-YP396026EIZ
CSB-EP396026EIZ
CSB-BP396026EIZ
CSB-MP396026EIZ
CSB-EP396026EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0181 protein Ent638_2380 (Ent638_2380) CSB-YP396027EIZ
CSB-EP396027EIZ
CSB-BP396027EIZ
CSB-MP396027EIZ
CSB-EP396027EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Protein mgrB homolog (mgrB), partial CSB-YP396028EIZ
CSB-EP396028EIZ
CSB-BP396028EIZ
CSB-MP396028EIZ
CSB-EP396028EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP396029EIZ
CSB-EP396029EIZ
CSB-BP396029EIZ
CSB-MP396029EIZ
CSB-EP396029EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Flagellar hook-basal body complex protein FliE (fliE) CSB-YP396030EIZ
CSB-EP396030EIZ
CSB-BP396030EIZ
CSB-MP396030EIZ
CSB-EP396030EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP396031EIZ
CSB-EP396031EIZ
CSB-BP396031EIZ
CSB-MP396031EIZ
CSB-EP396031EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Putative multidrug resistance protein MdtD (mdtD), partial CSB-YP396032EIZ
CSB-EP396032EIZ
CSB-BP396032EIZ
CSB-MP396032EIZ
CSB-EP396032EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Probable endonuclease 4 (nfo) CSB-YP396033EIZ
CSB-EP396033EIZ
CSB-BP396033EIZ
CSB-MP396033EIZ
CSB-EP396033EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP396034EIZ
CSB-EP396034EIZ
CSB-BP396034EIZ
CSB-MP396034EIZ
CSB-EP396034EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP396035EIZ
CSB-EP396035EIZ
CSB-BP396035EIZ
CSB-MP396035EIZ
CSB-EP396035EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP396036EIZ
CSB-EP396036EIZ
CSB-BP396036EIZ
CSB-MP396036EIZ
CSB-EP396036EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP396037EIZ
CSB-EP396037EIZ
CSB-BP396037EIZ
CSB-MP396037EIZ
CSB-EP396037EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Peptidase B (pepB) CSB-YP396038EIZ
CSB-EP396038EIZ
CSB-BP396038EIZ
CSB-MP396038EIZ
CSB-EP396038EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Autonomous glycyl radical cofactor (grcA) CSB-YP396039EIZ
CSB-EP396039EIZ
CSB-BP396039EIZ
CSB-MP396039EIZ
CSB-EP396039EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 30S ribosomal protein S16 (rpsP) CSB-YP396040EIZ
CSB-EP396040EIZ
CSB-BP396040EIZ
CSB-MP396040EIZ
CSB-EP396040EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Glutamate--cysteine ligase (gshA), partial CSB-YP396041EIZ
CSB-EP396041EIZ
CSB-BP396041EIZ
CSB-MP396041EIZ
CSB-EP396041EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Anaerobic nitric oxide reductase flavorubredoxin (norV) CSB-YP396042EIZ
CSB-EP396042EIZ
CSB-BP396042EIZ
CSB-MP396042EIZ
CSB-EP396042EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. tRNA pseudouridine synthase D (truD) CSB-YP396043EIZ
CSB-EP396043EIZ
CSB-BP396043EIZ
CSB-MP396043EIZ
CSB-EP396043EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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