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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Enterobacter sp. UPF0761 membrane protein Ent638_4092 (Ent638_4092), partial CSB-YP396064EIZ1
CSB-EP396064EIZ1
CSB-BP396064EIZ1
CSB-MP396064EIZ1
CSB-EP396064EIZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Membrane protein insertase YidC (yidC), partial CSB-YP396065EIZ1
CSB-EP396065EIZ1
CSB-BP396065EIZ1
CSB-MP396065EIZ1
CSB-EP396065EIZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Replication factor C large subunit (rfcL) CSB-YP396066PZU
CSB-EP396066PZU
CSB-BP396066PZU
CSB-MP396066PZU
CSB-EP396066PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum UPF0103 protein Pars_0062 (Pars_0062) CSB-YP396067PZU
CSB-EP396067PZU
CSB-BP396067PZU
CSB-MP396067PZU
CSB-EP396067PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Acylphosphatase (acyP) CSB-YP396068PZU
CSB-EP396068PZU
CSB-BP396068PZU
CSB-MP396068PZU
CSB-EP396068PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 50S ribosomal protein L30P (rpl30p) CSB-YP396069PZU
CSB-EP396069PZU
CSB-BP396069PZU
CSB-MP396069PZU
CSB-EP396069PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 30S ribosomal protein S3P (rps3p) CSB-YP396070PZU
CSB-EP396070PZU
CSB-BP396070PZU
CSB-MP396070PZU
CSB-EP396070PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Probable octanoyltransferase (lipB) CSB-YP396071PZU
CSB-EP396071PZU
CSB-BP396071PZU
CSB-MP396071PZU
CSB-EP396071PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Glutamyl-tRNA (Gln) amidotransferase subunit E, partial CSB-YP396072PZU
CSB-EP396072PZU
CSB-BP396072PZU
CSB-MP396072PZU
CSB-EP396072PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Translation initiation factor 6 (eif6) CSB-YP396073PZU
CSB-EP396073PZU
CSB-BP396073PZU
CSB-MP396073PZU
CSB-EP396073PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Orotate phosphoribosyltransferase (pyrE) CSB-YP396074PZU
CSB-EP396074PZU
CSB-BP396074PZU
CSB-MP396074PZU
CSB-EP396074PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 30S ribosomal protein S28e (rps28e) CSB-YP396075PZU
CSB-EP396075PZU
CSB-BP396075PZU
CSB-MP396075PZU
CSB-EP396075PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Prefoldin subunit beta (pfdB) CSB-YP396076PZU
CSB-EP396076PZU
CSB-BP396076PZU
CSB-MP396076PZU
CSB-EP396076PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Uracil phosphoribosyltransferase (upp) CSB-YP396077PZU
CSB-EP396077PZU
CSB-BP396077PZU
CSB-MP396077PZU
CSB-EP396077PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP396078PZU
CSB-EP396078PZU
CSB-BP396078PZU
CSB-MP396078PZU
CSB-EP396078PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 5-formaminoimidazole-4-carboxamide-1- (beta)-D-ribofuranosyl 5'-monophosphate synthetase CSB-YP396079PZU
CSB-EP396079PZU
CSB-BP396079PZU
CSB-MP396079PZU
CSB-EP396079PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S20 (rpsT) CSB-YP396080RLG
CSB-EP396080RLG
CSB-BP396080RLG
CSB-MP396080RLG
CSB-EP396080RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP396081RLG
CSB-EP396081RLG
CSB-BP396081RLG
CSB-MP396081RLG
CSB-EP396081RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides ATP synthase subunit c (atpE), partial CSB-YP396082RLG1
CSB-EP396082RLG1
CSB-BP396082RLG1
CSB-MP396082RLG1
CSB-EP396082RLG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP396083RLG1
CSB-EP396083RLG1
CSB-BP396083RLG1
CSB-MP396083RLG1
CSB-EP396083RLG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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