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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Caldicellulosiruptor saccharolyticus tRNA (guanine-N (1)-)-methyltransferase CSB-YP396164CVX
CSB-EP396164CVX
CSB-BP396164CVX
CSB-MP396164CVX
CSB-EP396164CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 30S ribosomal protein S4 (rpsD) CSB-YP396165CVX
CSB-EP396165CVX
CSB-BP396165CVX
CSB-MP396165CVX
CSB-EP396165CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Translation initiation factor IF-1 (infA) CSB-YP396166CVX
CSB-EP396166CVX
CSB-BP396166CVX
CSB-MP396166CVX
CSB-EP396166CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 50S ribosomal protein L14 (rplN) CSB-YP396167CVX
CSB-EP396167CVX
CSB-BP396167CVX
CSB-MP396167CVX
CSB-EP396167CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 30S ribosomal protein S3 (rpsC) CSB-YP396168CVX
CSB-EP396168CVX
CSB-BP396168CVX
CSB-MP396168CVX
CSB-EP396168CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 30S ribosomal protein S10 (rpsJ) CSB-YP396169CVX
CSB-EP396169CVX
CSB-BP396169CVX
CSB-MP396169CVX
CSB-EP396169CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina DNA replication and repair protein recF (recF) CSB-YP396170PZM
CSB-EP396170PZM
CSB-BP396170PZM
CSB-MP396170PZM
CSB-EP396170PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP396171PZM
CSB-EP396171PZM
CSB-BP396171PZM
CSB-MP396171PZM
CSB-EP396171PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Urease subunit gamma (ureA) CSB-YP396172YAL
CSB-EP396172YAL
CSB-BP396172YAL
CSB-MP396172YAL
CSB-EP396172YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP396173YAL
CSB-EP396173YAL
CSB-BP396173YAL
CSB-MP396173YAL
CSB-EP396173YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP396174YAL
CSB-EP396174YAL
CSB-BP396174YAL
CSB-MP396174YAL
CSB-EP396174YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Lysophospholipid transporter lplT (lplT), partial CSB-YP396175YAL
CSB-EP396175YAL
CSB-BP396175YAL
CSB-MP396175YAL
CSB-EP396175YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0227 protein YPDSF_1831 (YPDSF_1831) CSB-YP396176YAL
CSB-EP396176YAL
CSB-BP396176YAL
CSB-MP396176YAL
CSB-EP396176YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0303 protein YPDSF_1944 (YPDSF_1944) CSB-YP396177YAL
CSB-EP396177YAL
CSB-BP396177YAL
CSB-MP396177YAL
CSB-EP396177YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0208 membrane protein YPDSF_1972 (YPDSF_1972), partial CSB-YP396178YAL1
CSB-EP396178YAL1
CSB-BP396178YAL1
CSB-MP396178YAL1
CSB-EP396178YAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP396179YAL
CSB-EP396179YAL
CSB-BP396179YAL
CSB-MP396179YAL
CSB-EP396179YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Cytoskeleton protein rodZ (rodZ), partial CSB-YP396180YAL
CSB-EP396180YAL
CSB-BP396180YAL
CSB-MP396180YAL
CSB-EP396180YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ CSB-YP396181YAL
CSB-EP396181YAL
CSB-BP396181YAL
CSB-MP396181YAL
CSB-EP396181YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Chromosome partition protein MukB (mukB), partial CSB-YP396182YAL
CSB-EP396182YAL
CSB-BP396182YAL
CSB-MP396182YAL
CSB-EP396182YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Uncharacterized MFS-type transporter YPDSF_2315 (YPDSF_2315), partial CSB-YP396183YAL
CSB-EP396183YAL
CSB-BP396183YAL
CSB-MP396183YAL
CSB-EP396183YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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