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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas stutzeri Cytidylate kinase (cmk) CSB-YP396244PZP
CSB-EP396244PZP
CSB-BP396244PZP
CSB-MP396244PZP
CSB-EP396244PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Threonine--tRNA ligase (thrS), partial CSB-YP396245PZP
CSB-EP396245PZP
CSB-BP396245PZP
CSB-MP396245PZP
CSB-EP396245PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Elongation factor P (efp) CSB-YP396246PZP
CSB-EP396246PZP
CSB-BP396246PZP
CSB-MP396246PZP
CSB-EP396246PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Na (+)-translocating NADH-quinone reductase subunit B, partial CSB-YP396247PZP
CSB-EP396247PZP
CSB-BP396247PZP
CSB-MP396247PZP
CSB-EP396247PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP396248PZP
CSB-EP396248PZP
CSB-BP396248PZP
CSB-MP396248PZP
CSB-EP396248PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Glutamate--tRNA ligase (gltX) CSB-YP396249PZP
CSB-EP396249PZP
CSB-BP396249PZP
CSB-MP396249PZP
CSB-EP396249PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 2-isopropylmalate synthase (leuA), partial CSB-YP396250PZP
CSB-EP396250PZP
CSB-BP396250PZP
CSB-MP396250PZP
CSB-EP396250PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Protein translocase subunit SecA (secA), partial CSB-YP396251PZP
CSB-EP396251PZP
CSB-BP396251PZP
CSB-MP396251PZP
CSB-EP396251PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Aspartate 1-decarboxylase (panD) CSB-YP396252PZP
CSB-EP396252PZP
CSB-BP396252PZP
CSB-MP396252PZP
CSB-EP396252PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Putative pterin-4-alpha-carbinolamine dehydratase (PST_3563) CSB-YP396253PZP
CSB-EP396253PZP
CSB-BP396253PZP
CSB-MP396253PZP
CSB-EP396253PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP396254PZP
CSB-EP396254PZP
CSB-BP396254PZP
CSB-MP396254PZP
CSB-EP396254PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP396255PZP
CSB-EP396255PZP
CSB-BP396255PZP
CSB-MP396255PZP
CSB-EP396255PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 8-amino-7-oxononanoate synthase (bioF) CSB-YP396256PZP
CSB-EP396256PZP
CSB-BP396256PZP
CSB-MP396256PZP
CSB-EP396256PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Probable Fe (2+)-trafficking protein CSB-YP396257PZP
CSB-EP396257PZP
CSB-BP396257PZP
CSB-MP396257PZP
CSB-EP396257PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Putative competence-damage inducible protein (cinA) CSB-YP396258SUS
CSB-EP396258SUS
CSB-BP396258SUS
CSB-MP396258SUS
CSB-EP396258SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L29 (rpmC) CSB-YP396259SUS
CSB-EP396259SUS
CSB-BP396259SUS
CSB-MP396259SUS
CSB-EP396259SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 30S ribosomal protein S13 (rpsM) CSB-YP396260SUS
CSB-EP396260SUS
CSB-BP396260SUS
CSB-MP396260SUS
CSB-EP396260SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 30S ribosomal protein S7 (rpsG) CSB-YP396261SUS
CSB-EP396261SUS
CSB-BP396261SUS
CSB-MP396261SUS
CSB-EP396261SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Protein grpE (grpE) CSB-YP396262SUS
CSB-EP396262SUS
CSB-BP396262SUS
CSB-MP396262SUS
CSB-EP396262SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Trigger factor (tig) CSB-YP396263SUS
CSB-EP396263SUS
CSB-BP396263SUS
CSB-MP396263SUS
CSB-EP396263SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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