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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bradyrhizobium sp. S-adenosylmethionine synthase (metK) CSB-YP397065BPF
CSB-EP397065BPF
CSB-BP397065BPF
CSB-MP397065BPF
CSB-EP397065BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UPF0391 membrane protein BRADO5617 (BRADO5617), partial CSB-YP397066BPF
CSB-EP397066BPF
CSB-BP397066BPF
CSB-MP397066BPF
CSB-EP397066BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Phosphomethylpyrimidine synthase (thiC), partial CSB-YP397067BPF
CSB-EP397067BPF
CSB-BP397067BPF
CSB-MP397067BPF
CSB-EP397067BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Peptidyl-tRNA hydrolase (pth) CSB-YP397068BPF
CSB-EP397068BPF
CSB-BP397068BPF
CSB-MP397068BPF
CSB-EP397068BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UPF0753 protein BRADO6223 (BRADO6223), partial CSB-YP397069BPF
CSB-EP397069BPF
CSB-BP397069BPF
CSB-MP397069BPF
CSB-EP397069BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Protein VP3, partial CSB-YP397070ROC
CSB-EP397070ROC
CSB-BP397070ROC
CSB-MP397070ROC
CSB-EP397070ROC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF99b (ORF99b) CSB-YP397071ATY
CSB-EP397071ATY
CSB-BP397071ATY
CSB-MP397071ATY
CSB-EP397071ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Putative thymidylate kinase (ORF156) CSB-YP397072ATY
CSB-EP397072ATY
CSB-BP397072ATY
CSB-MP397072ATY
CSB-EP397072ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF53b (ORF53b), partial CSB-YP397073ATY
CSB-EP397073ATY
CSB-BP397073ATY
CSB-MP397073ATY
CSB-EP397073ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF86 (ORF86) CSB-YP397074ATY
CSB-EP397074ATY
CSB-BP397074ATY
CSB-MP397074ATY
CSB-EP397074ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Putative transmembrane protein ORF85 (ORF85), partial CSB-YP397075ATY1
CSB-EP397075ATY1
CSB-BP397075ATY1
CSB-MP397075ATY1
CSB-EP397075ATY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF54 (ORF54), partial CSB-YP397076ATY
CSB-EP397076ATY
CSB-BP397076ATY
CSB-MP397076ATY
CSB-EP397076ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF57 (ORF57), partial CSB-YP397077ATY
CSB-EP397077ATY
CSB-BP397077ATY
CSB-MP397077ATY
CSB-EP397077ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Atrax robustus Omega-hexatoxin-Ar1d CSB-YP397078AVG
CSB-EP397078AVG
CSB-BP397078AVG
CSB-MP397078AVG
CSB-EP397078AVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ydgU (ydgU) CSB-YP397079ENV
CSB-EP397079ENV
CSB-BP397079ENV
CSB-MP397079ENV
CSB-EP397079ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yjbT (yjbT) CSB-YP397080ENV
CSB-EP397080ENV
CSB-BP397080ENV
CSB-MP397080ENV
CSB-EP397080ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes RNA-binding motif protein, X chromosome (RBMX) CSB-YP397081EQV
CSB-EP397081EQV
CSB-BP397081EQV
CSB-MP397081EQV
CSB-EP397081EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Calcium-dependent protein kinase 5 (CPK5), partial CSB-YP397082FIG
CSB-EP397082FIG
CSB-BP397082FIG
CSB-MP397082FIG
CSB-EP397082FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger ATPase get3 (get3) CSB-YP397083AVE
CSB-EP397083AVE
CSB-BP397083AVE
CSB-MP397083AVE
CSB-EP397083AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Lipoyl synthase, mitochondrial (An11g09760) CSB-YP397084AVE
CSB-EP397084AVE
CSB-BP397084AVE
CSB-MP397084AVE
CSB-EP397084AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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