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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Metallosphaera sedula Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP397626MNM
CSB-EP397626MNM
CSB-BP397626MNM
CSB-MP397626MNM
CSB-EP397626MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 50S ribosomal protein L10e (rpl10e) CSB-YP397627MNM
CSB-EP397627MNM
CSB-BP397627MNM
CSB-MP397627MNM
CSB-EP397627MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Digeranylgeranylglyceryl phosphate synthase (Msed_1933), partial CSB-YP397628MNM1
CSB-EP397628MNM1
CSB-BP397628MNM1
CSB-MP397628MNM1
CSB-EP397628MNM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP397629MNM
CSB-EP397629MNM
CSB-BP397629MNM
CSB-MP397629MNM
CSB-EP397629MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula UPF0173 metal-dependent hydrolase Msed_2125 (Msed_2125) CSB-YP397630MNM
CSB-EP397630MNM
CSB-BP397630MNM
CSB-MP397630MNM
CSB-EP397630MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 30S ribosomal protein S4P (rps4p) CSB-YP397631MNM
CSB-EP397631MNM
CSB-BP397631MNM
CSB-MP397631MNM
CSB-EP397631MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Protein translation factor SUI1 homolog (Msed_2210) CSB-YP397632MNM
CSB-EP397632MNM
CSB-BP397632MNM
CSB-MP397632MNM
CSB-EP397632MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Proteasome subunit beta 2 (psmB2) CSB-YP397633MNM
CSB-EP397633MNM
CSB-BP397633MNM
CSB-MP397633MNM
CSB-EP397633MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP397634BPF
CSB-EP397634BPF
CSB-BP397634BPF
CSB-MP397634BPF
CSB-EP397634BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Tryptophan synthase alpha chain (trpA) CSB-YP397635BPF
CSB-EP397635BPF
CSB-BP397635BPF
CSB-MP397635BPF
CSB-EP397635BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP397636BPF
CSB-EP397636BPF
CSB-BP397636BPF
CSB-MP397636BPF
CSB-EP397636BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Dihydrodipicolinate reductase (dapB) CSB-YP397637BPF
CSB-EP397637BPF
CSB-BP397637BPF
CSB-MP397637BPF
CSB-EP397637BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ribonuclease PH (rph) CSB-YP397638BPF
CSB-EP397638BPF
CSB-BP397638BPF
CSB-MP397638BPF
CSB-EP397638BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP397639BPF
CSB-EP397639BPF
CSB-BP397639BPF
CSB-MP397639BPF
CSB-EP397639BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP397640BPF
CSB-EP397640BPF
CSB-BP397640BPF
CSB-MP397640BPF
CSB-EP397640BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L32 (rpmF) CSB-YP397641BPF
CSB-EP397641BPF
CSB-BP397641BPF
CSB-MP397641BPF
CSB-EP397641BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Recombination protein RecR (recR) CSB-YP397642BPF
CSB-EP397642BPF
CSB-BP397642BPF
CSB-MP397642BPF
CSB-EP397642BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L34 (rpmH) CSB-YP397643BPF
CSB-EP397643BPF
CSB-BP397643BPF
CSB-MP397643BPF
CSB-EP397643BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Urease accessory protein UreF (ureF) CSB-YP397644BPF
CSB-EP397644BPF
CSB-BP397644BPF
CSB-MP397644BPF
CSB-EP397644BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Gas vesicle structural protein (gvpA) CSB-YP397645BPF
CSB-EP397645BPF
CSB-BP397645BPF
CSB-MP397645BPF
CSB-EP397645BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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