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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clavibacter michiganensis subsp. michiganensis Bifunctional enzyme IspD/IspF (ispDF) CSB-YP397506CWT
CSB-EP397506CWT
CSB-BP397506CWT
CSB-MP397506CWT
CSB-EP397506CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP397507CWT
CSB-EP397507CWT
CSB-BP397507CWT
CSB-MP397507CWT
CSB-EP397507CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 50S ribosomal protein L14 (rplN) CSB-YP397508CWT
CSB-EP397508CWT
CSB-BP397508CWT
CSB-MP397508CWT
CSB-EP397508CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 30S ribosomal protein S7 (rpsG) CSB-YP397509CWT
CSB-EP397509CWT
CSB-BP397509CWT
CSB-MP397509CWT
CSB-EP397509CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Deoxycytidine triphosphate deaminase (dcd) CSB-YP397510CWT
CSB-EP397510CWT
CSB-BP397510CWT
CSB-MP397510CWT
CSB-EP397510CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Ribonuclease P protein component (rnpA) CSB-YP397511CWT
CSB-EP397511CWT
CSB-BP397511CWT
CSB-MP397511CWT
CSB-EP397511CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Protein CyaY (cyaY) CSB-YP397512PZM
CSB-EP397512PZM
CSB-BP397512PZM
CSB-MP397512PZM
CSB-EP397512PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Betaine aldehyde dehydrogenase (betB) CSB-YP397513PZM
CSB-EP397513PZM
CSB-BP397513PZM
CSB-MP397513PZM
CSB-EP397513PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Phosphomethylpyrimidine synthase (thiC), partial CSB-YP397514PZM
CSB-EP397514PZM
CSB-BP397514PZM
CSB-MP397514PZM
CSB-EP397514PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP397515PZM
CSB-EP397515PZM
CSB-BP397515PZM
CSB-MP397515PZM
CSB-EP397515PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP397516PZM
CSB-EP397516PZM
CSB-BP397516PZM
CSB-MP397516PZM
CSB-EP397516PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 30S ribosomal protein S9 (rpsI) CSB-YP397517PZM
CSB-EP397517PZM
CSB-BP397517PZM
CSB-MP397517PZM
CSB-EP397517PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Protein MraZ (mraZ) CSB-YP397518PZM
CSB-EP397518PZM
CSB-BP397518PZM
CSB-MP397518PZM
CSB-EP397518PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP397519PZM
CSB-EP397519PZM
CSB-BP397519PZM
CSB-MP397519PZM
CSB-EP397519PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina UPF0246 protein Pmen_1032 (Pmen_1032) CSB-YP397520PZM
CSB-EP397520PZM
CSB-BP397520PZM
CSB-MP397520PZM
CSB-EP397520PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP397521PZM
CSB-EP397521PZM
CSB-BP397521PZM
CSB-MP397521PZM
CSB-EP397521PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Probable 5-dehydro-4-deoxyglucarate dehydratase (Pmen_1215) CSB-YP397522PZM
CSB-EP397522PZM
CSB-BP397522PZM
CSB-MP397522PZM
CSB-EP397522PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Electron transport complex protein RnfG (rnfG), partial CSB-YP397523PZM1
CSB-EP397523PZM1
CSB-BP397523PZM1
CSB-MP397523PZM1
CSB-EP397523PZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Electron transport complex protein RnfB (rnfB) CSB-YP397524PZM
CSB-EP397524PZM
CSB-BP397524PZM
CSB-MP397524PZM
CSB-EP397524PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP397525PZM
CSB-EP397525PZM
CSB-BP397525PZM
CSB-MP397525PZM
CSB-EP397525PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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