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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio cholerae serotype O1 Outer membrane protein U (ompU) CSB-YP398706VEY
CSB-EP398706VEY
CSB-BP398706VEY
CSB-MP398706VEY
CSB-EP398706VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Pantothenate synthetase (panC) CSB-YP398707VEY
CSB-EP398707VEY
CSB-BP398707VEY
CSB-MP398707VEY
CSB-EP398707VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Penicillin-binding protein activator LpoA (lpoA), partial CSB-YP398708VEY
CSB-EP398708VEY
CSB-BP398708VEY
CSB-MP398708VEY
CSB-EP398708VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 tRNA (guanine-N (1)-)-methyltransferase CSB-YP398709VEY
CSB-EP398709VEY
CSB-BP398709VEY
CSB-MP398709VEY
CSB-EP398709VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 DNA mismatch repair protein MutS (mutS), partial CSB-YP398710VEY
CSB-EP398710VEY
CSB-BP398710VEY
CSB-MP398710VEY
CSB-EP398710VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP398711VEY
CSB-EP398711VEY
CSB-BP398711VEY
CSB-MP398711VEY
CSB-EP398711VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 S-adenosylmethionine synthase (metK) CSB-YP398712VEY
CSB-EP398712VEY
CSB-BP398712VEY
CSB-MP398712VEY
CSB-EP398712VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae CinA-like protein (Fjoh_4984) CSB-YP398713FDT
CSB-EP398713FDT
CSB-BP398713FDT
CSB-MP398713FDT
CSB-EP398713FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Proline--tRNA ligase (proS) CSB-YP398714FDT
CSB-EP398714FDT
CSB-BP398714FDT
CSB-MP398714FDT
CSB-EP398714FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Urease accessory protein UreE (ureE) CSB-YP398715FDT
CSB-EP398715FDT
CSB-BP398715FDT
CSB-MP398715FDT
CSB-EP398715FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Urease accessory protein UreD (ureD) CSB-YP398716FDT
CSB-EP398716FDT
CSB-BP398716FDT
CSB-MP398716FDT
CSB-EP398716FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Imidazolonepropionase (hutI) CSB-YP398717FDT
CSB-EP398717FDT
CSB-BP398717FDT
CSB-MP398717FDT
CSB-EP398717FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Uronate isomerase (uxaC) CSB-YP398718FDT
CSB-EP398718FDT
CSB-BP398718FDT
CSB-MP398718FDT
CSB-EP398718FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae SsrA-binding protein (smpB) CSB-YP398719FDT
CSB-EP398719FDT
CSB-BP398719FDT
CSB-MP398719FDT
CSB-EP398719FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Histidinol-phosphate aminotransferase (hisC) CSB-YP398720FDT
CSB-EP398720FDT
CSB-BP398720FDT
CSB-MP398720FDT
CSB-EP398720FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP398721FDT
CSB-EP398721FDT
CSB-BP398721FDT
CSB-MP398721FDT
CSB-EP398721FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae UPF0133 protein Fjoh_2555 (Fjoh_2555) CSB-YP398722FDT
CSB-EP398722FDT
CSB-BP398722FDT
CSB-MP398722FDT
CSB-EP398722FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Dihydroorotate dehydrogenase (quinone) CSB-YP398723FDT
CSB-EP398723FDT
CSB-BP398723FDT
CSB-MP398723FDT
CSB-EP398723FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L11 (rplK) CSB-YP398724FDT
CSB-EP398724FDT
CSB-BP398724FDT
CSB-MP398724FDT
CSB-EP398724FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Ribonuclease HII (rnhB) CSB-YP398725FDT
CSB-EP398725FDT
CSB-BP398725FDT
CSB-MP398725FDT
CSB-EP398725FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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