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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vibrio cholerae serotype O1 Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP398646VEY
CSB-EP398646VEY
CSB-BP398646VEY
CSB-MP398646VEY
CSB-EP398646VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Cobalamin synthase (cobS), partial CSB-YP398647VEY1
CSB-EP398647VEY1
CSB-BP398647VEY1
CSB-MP398647VEY1
CSB-EP398647VEY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UPF0299 membrane protein VC0395_A0854/VC395_1352 (VC0395_A0854, VC395_1352), partial CSB-YP398648VEY1
CSB-EP398648VEY1
CSB-BP398648VEY1
CSB-MP398648VEY1
CSB-EP398648VEY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Tryptophan synthase alpha chain (trpA) CSB-YP398649VEY
CSB-EP398649VEY
CSB-BP398649VEY
CSB-MP398649VEY
CSB-EP398649VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Putative transport protein VC0395_A0715/VC395_1212 (VC0395_A0715, VC395_1212), partial CSB-YP398650VEY
CSB-EP398650VEY
CSB-BP398650VEY
CSB-MP398650VEY
CSB-EP398650VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP398651VEY
CSB-EP398651VEY
CSB-BP398651VEY
CSB-MP398651VEY
CSB-EP398651VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP398652VEY
CSB-EP398652VEY
CSB-BP398652VEY
CSB-MP398652VEY
CSB-EP398652VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Electron transport complex protein RnfE (rnfE), partial CSB-YP398653VEY1
CSB-EP398653VEY1
CSB-BP398653VEY1
CSB-MP398653VEY1
CSB-EP398653VEY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Cell division protein ZipA homolog (zipA), partial CSB-YP398654VEY
CSB-EP398654VEY
CSB-BP398654VEY
CSB-MP398654VEY
CSB-EP398654VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Leucine--tRNA ligase (leuS), partial CSB-YP398655VEY
CSB-EP398655VEY
CSB-BP398655VEY
CSB-MP398655VEY
CSB-EP398655VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Octanoyltransferase (lipB) CSB-YP398656VEY
CSB-EP398656VEY
CSB-BP398656VEY
CSB-MP398656VEY
CSB-EP398656VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP398657VEY
CSB-EP398657VEY
CSB-BP398657VEY
CSB-MP398657VEY
CSB-EP398657VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Membrane-bound lytic murein transglycosylase F (mltF), partial CSB-YP398658VEY
CSB-EP398658VEY
CSB-BP398658VEY
CSB-MP398658VEY
CSB-EP398658VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP398659VEY
CSB-EP398659VEY
CSB-BP398659VEY
CSB-MP398659VEY
CSB-EP398659VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 SsrA-binding protein (smpB) CSB-YP398660VEY
CSB-EP398660VEY
CSB-BP398660VEY
CSB-MP398660VEY
CSB-EP398660VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 DNA repair protein recN (recN), partial CSB-YP398661VEY
CSB-EP398661VEY
CSB-BP398661VEY
CSB-MP398661VEY
CSB-EP398661VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Toxin coregulated pilus biosynthesis protein I (tcpI), partial CSB-YP398662VEY
CSB-EP398662VEY
CSB-BP398662VEY
CSB-MP398662VEY
CSB-EP398662VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Probable apo-citrate lyase phosphoribosyl-dephospho-CoA transferase (citX) CSB-YP398663VEY
CSB-EP398663VEY
CSB-BP398663VEY
CSB-MP398663VEY
CSB-EP398663VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP398664VEY
CSB-EP398664VEY
CSB-BP398664VEY
CSB-MP398664VEY
CSB-EP398664VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP398665VEY
CSB-EP398665VEY
CSB-BP398665VEY
CSB-MP398665VEY
CSB-EP398665VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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