Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vibrio cholerae serotype O1 tRNA (Ile)-lysidine synthase CSB-YP398686VEY
CSB-EP398686VEY
CSB-BP398686VEY
CSB-MP398686VEY
CSB-EP398686VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Ribonuclease H (rnhA) CSB-YP398687VEY
CSB-EP398687VEY
CSB-BP398687VEY
CSB-MP398687VEY
CSB-EP398687VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Glutamate--tRNA ligase (gltX) CSB-YP398688VEY
CSB-EP398688VEY
CSB-BP398688VEY
CSB-MP398688VEY
CSB-EP398688VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Flagellar L-ring protein (flgH) CSB-YP398689VEY
CSB-EP398689VEY
CSB-BP398689VEY
CSB-MP398689VEY
CSB-EP398689VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Dihydrodipicolinate synthase (dapA) CSB-YP398690VEY
CSB-EP398690VEY
CSB-BP398690VEY
CSB-MP398690VEY
CSB-EP398690VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Flagellin B (flaB) CSB-YP398691VEY
CSB-EP398691VEY
CSB-BP398691VEY
CSB-MP398691VEY
CSB-EP398691VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP398692VEY
CSB-EP398692VEY
CSB-BP398692VEY
CSB-MP398692VEY
CSB-EP398692VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP398693VEY
CSB-EP398693VEY
CSB-BP398693VEY
CSB-MP398693VEY
CSB-EP398693VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP398694VEY
CSB-EP398694VEY
CSB-BP398694VEY
CSB-MP398694VEY
CSB-EP398694VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UPF0434 protein VC0395_A1467/VC395_1991 (VC0395_A1467, VC395_1991) CSB-YP398695VEY
CSB-EP398695VEY
CSB-BP398695VEY
CSB-MP398695VEY
CSB-EP398695VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP398696VEY
CSB-EP398696VEY
CSB-BP398696VEY
CSB-MP398696VEY
CSB-EP398696VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Quinolinate synthase A (nadA) CSB-YP398697VEY
CSB-EP398697VEY
CSB-BP398697VEY
CSB-MP398697VEY
CSB-EP398697VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Putative arginyl-tRNA--protein transferase (ate) CSB-YP398698VEY
CSB-EP398698VEY
CSB-BP398698VEY
CSB-MP398698VEY
CSB-EP398698VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 HTH-type transcriptional repressor PurR (purR) CSB-YP398699VEY
CSB-EP398699VEY
CSB-BP398699VEY
CSB-MP398699VEY
CSB-EP398699VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 50S ribosomal protein L25 (rplY) CSB-YP398700VEY
CSB-EP398700VEY
CSB-BP398700VEY
CSB-MP398700VEY
CSB-EP398700VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP398701VEY
CSB-EP398701VEY
CSB-BP398701VEY
CSB-MP398701VEY
CSB-EP398701VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP398702VEY
CSB-EP398702VEY
CSB-BP398702VEY
CSB-MP398702VEY
CSB-EP398702VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Copper homeostasis protein CutC (cutC) CSB-YP398703VEY
CSB-EP398703VEY
CSB-BP398703VEY
CSB-MP398703VEY
CSB-EP398703VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Isoleucine--tRNA ligase (ileS), partial CSB-YP398704VEY
CSB-EP398704VEY
CSB-BP398704VEY
CSB-MP398704VEY
CSB-EP398704VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 tRNA pseudouridine synthase B (truB) CSB-YP398705VEY
CSB-EP398705VEY
CSB-BP398705VEY
CSB-MP398705VEY
CSB-EP398705VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>