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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Legionella pneumophila Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP400927LLJ
CSB-EP400927LLJ
CSB-BP400927LLJ
CSB-MP400927LLJ
CSB-EP400927LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 50S ribosomal protein L9 (rplI) CSB-YP400928LLJ
CSB-EP400928LLJ
CSB-BP400928LLJ
CSB-MP400928LLJ
CSB-EP400928LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Translation initiation factor IF-1 (infA) CSB-YP400929LLJ
CSB-EP400929LLJ
CSB-BP400929LLJ
CSB-MP400929LLJ
CSB-EP400929LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP400930LLJ
CSB-EP400930LLJ
CSB-BP400930LLJ
CSB-MP400930LLJ
CSB-EP400930LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Trigger factor (tig) CSB-YP400931LLJ
CSB-EP400931LLJ
CSB-BP400931LLJ
CSB-MP400931LLJ
CSB-EP400931LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Putative pterin-4-alpha-carbinolamine dehydratase (LPC_1482) CSB-YP400932LLJ
CSB-EP400932LLJ
CSB-BP400932LLJ
CSB-MP400932LLJ
CSB-EP400932LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Asparagine--tRNA ligase (asnS) CSB-YP400933LLJ
CSB-EP400933LLJ
CSB-BP400933LLJ
CSB-MP400933LLJ
CSB-EP400933LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Glutamyl-tRNA reductase (hemA) CSB-YP400934LLJ
CSB-EP400934LLJ
CSB-BP400934LLJ
CSB-MP400934LLJ
CSB-EP400934LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Probable potassium transport system protein kup 3 (kup3), partial CSB-YP400935LLJ
CSB-EP400935LLJ
CSB-BP400935LLJ
CSB-MP400935LLJ
CSB-EP400935LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Indole-3-glycerol phosphate synthase (trpC) CSB-YP400936LLJ
CSB-EP400936LLJ
CSB-BP400936LLJ
CSB-MP400936LLJ
CSB-EP400936LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP400937LLJ
CSB-EP400937LLJ
CSB-BP400937LLJ
CSB-MP400937LLJ
CSB-EP400937LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Phosphopentomutase (deoB) CSB-YP400938LLJ
CSB-EP400938LLJ
CSB-BP400938LLJ
CSB-MP400938LLJ
CSB-EP400938LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Phosphopantetheine adenylyltransferase (coaD) CSB-YP400939LLJ
CSB-EP400939LLJ
CSB-BP400939LLJ
CSB-MP400939LLJ
CSB-EP400939LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Protein CrcB homolog (crcB), partial CSB-YP400940LLJ1
CSB-EP400940LLJ1
CSB-BP400940LLJ1
CSB-MP400940LLJ1
CSB-EP400940LLJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 50S ribosomal protein L28 (rpmB) CSB-YP400941LLJ
CSB-EP400941LLJ
CSB-BP400941LLJ
CSB-MP400941LLJ
CSB-EP400941LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 50S ribosomal protein L17 (rplQ) CSB-YP400942LLJ
CSB-EP400942LLJ
CSB-BP400942LLJ
CSB-MP400942LLJ
CSB-EP400942LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 30S ribosomal protein S8 (rpsH) CSB-YP400943LLJ
CSB-EP400943LLJ
CSB-BP400943LLJ
CSB-MP400943LLJ
CSB-EP400943LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 30S ribosomal protein S3 (rpsC) CSB-YP400944LLJ
CSB-EP400944LLJ
CSB-BP400944LLJ
CSB-MP400944LLJ
CSB-EP400944LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 50S ribosomal protein L11 (rplK) CSB-YP400945LLJ
CSB-EP400945LLJ
CSB-BP400945LLJ
CSB-MP400945LLJ
CSB-EP400945LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP400946LLJ
CSB-EP400946LLJ
CSB-BP400946LLJ
CSB-MP400946LLJ
CSB-EP400946LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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