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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella pealeana Ribosome maturation factor RimM (rimM) CSB-YP424798STP
CSB-EP424798STP
CSB-BP424798STP
CSB-MP424798STP
CSB-EP424798STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 50S ribosomal protein L19 (rplS) CSB-YP424799STP
CSB-EP424799STP
CSB-BP424799STP
CSB-MP424799STP
CSB-EP424799STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana tRNA (guanine-N (7)-)-methyltransferase CSB-YP424800STP
CSB-EP424800STP
CSB-BP424800STP
CSB-MP424800STP
CSB-EP424800STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP424801STP
CSB-EP424801STP
CSB-BP424801STP
CSB-MP424801STP
CSB-EP424801STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Glutamate--cysteine ligase (gshA), partial CSB-YP424802STP
CSB-EP424802STP
CSB-BP424802STP
CSB-MP424802STP
CSB-EP424802STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Histidine--tRNA ligase (hisS) CSB-YP424803STP
CSB-EP424803STP
CSB-BP424803STP
CSB-MP424803STP
CSB-EP424803STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Glycosyl hydrolase family 109 protein (Spea_1465) CSB-YP424804STP
CSB-EP424804STP
CSB-BP424804STP
CSB-MP424804STP
CSB-EP424804STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana N-succinylarginine dihydrolase (astB) CSB-YP424805STP
CSB-EP424805STP
CSB-BP424805STP
CSB-MP424805STP
CSB-EP424805STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Acetate kinase (ackA) CSB-YP424806STP
CSB-EP424806STP
CSB-BP424806STP
CSB-MP424806STP
CSB-EP424806STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana UPF0434 protein Spea_1772 (Spea_1772) CSB-YP424807STP
CSB-EP424807STP
CSB-BP424807STP
CSB-MP424807STP
CSB-EP424807STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP424808STP
CSB-EP424808STP
CSB-BP424808STP
CSB-MP424808STP
CSB-EP424808STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Dihydrodipicolinate synthase (dapA) CSB-YP424809STP
CSB-EP424809STP
CSB-BP424809STP
CSB-MP424809STP
CSB-EP424809STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP424810STP
CSB-EP424810STP
CSB-BP424810STP
CSB-MP424810STP
CSB-EP424810STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 4-hydroxy-2-oxovalerate aldolase (Spea_2116) CSB-YP424811STP
CSB-EP424811STP
CSB-BP424811STP
CSB-MP424811STP
CSB-EP424811STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 50S ribosomal protein L35 (rpmI) CSB-YP424812STP
CSB-EP424812STP
CSB-BP424812STP
CSB-MP424812STP
CSB-EP424812STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Electron transport complex protein RnfE (rnfE), partial CSB-YP424813STP1
CSB-EP424813STP1
CSB-BP424813STP1
CSB-MP424813STP1
CSB-EP424813STP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Aspartate--tRNA ligase (aspS) CSB-YP424814STP
CSB-EP424814STP
CSB-BP424814STP
CSB-MP424814STP
CSB-EP424814STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Na (+)/H (+) antiporter nhaB, partial CSB-YP424815STP
CSB-EP424815STP
CSB-BP424815STP
CSB-MP424815STP
CSB-EP424815STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP424816STP
CSB-EP424816STP
CSB-BP424816STP
CSB-MP424816STP
CSB-EP424816STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana UPF0502 protein Spea_2482 (Spea_2482) CSB-YP424817STP
CSB-EP424817STP
CSB-BP424817STP
CSB-MP424817STP
CSB-EP424817STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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