Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O9:H4 Multifunctional CCA protein (cca) CSB-YP425199EJF
CSB-EP425199EJF
CSB-BP425199EJF
CSB-MP425199EJF
CSB-EP425199EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Uronate isomerase (uxaC) CSB-YP425200EJF
CSB-EP425200EJF
CSB-BP425200EJF
CSB-MP425200EJF
CSB-EP425200EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 UPF0213 protein yhbQ (yhbQ) CSB-YP425201EJF
CSB-EP425201EJF
CSB-BP425201EJF
CSB-MP425201EJF
CSB-EP425201EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Phosphoglucosamine mutase (glmM) CSB-YP425202EJF
CSB-EP425202EJF
CSB-BP425202EJF
CSB-MP425202EJF
CSB-EP425202EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 N-acetylmannosamine kinase (nanK) CSB-YP425203EJF
CSB-EP425203EJF
CSB-BP425203EJF
CSB-MP425203EJF
CSB-EP425203EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 p-hydroxybenzoic acid efflux pump subunit AaeB (aaeB), partial CSB-YP425204EJF
CSB-EP425204EJF
CSB-BP425204EJF
CSB-MP425204EJF
CSB-EP425204EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 50S ribosomal protein L15 (rplO) CSB-YP425205EJF
CSB-EP425205EJF
CSB-BP425205EJF
CSB-MP425205EJF
CSB-EP425205EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 30S ribosomal protein S3 (rpsC) CSB-YP425206EJF
CSB-EP425206EJF
CSB-BP425206EJF
CSB-MP425206EJF
CSB-EP425206EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Glutathione-regulated potassium-efflux system ancillary protein KefG (kefG) CSB-YP425207EJF
CSB-EP425207EJF
CSB-BP425207EJF
CSB-MP425207EJF
CSB-EP425207EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Fe/S biogenesis protein NfuA (nfuA) CSB-YP425208EJF
CSB-EP425208EJF
CSB-BP425208EJF
CSB-MP425208EJF
CSB-EP425208EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 1,4-alpha-glucan branching enzyme GlgB (glgB) CSB-YP425209EJF
CSB-EP425209EJF
CSB-BP425209EJF
CSB-MP425209EJF
CSB-EP425209EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Glyoxylate/hydroxypyruvate reductase B (ghrB) CSB-YP425210EJF
CSB-EP425210EJF
CSB-BP425210EJF
CSB-MP425210EJF
CSB-EP425210EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP425211EJF
CSB-EP425211EJF
CSB-BP425211EJF
CSB-MP425211EJF
CSB-EP425211EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 UPF0758 protein yicR (yicR) CSB-YP425212EJF
CSB-EP425212EJF
CSB-BP425212EJF
CSB-MP425212EJF
CSB-EP425212EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Putative transport protein YidE (yidE), partial CSB-YP425213EJF
CSB-EP425213EJF
CSB-BP425213EJF
CSB-MP425213EJF
CSB-EP425213EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Putative membrane protein insertion efficiency factor (yidD) CSB-YP425214EJF
CSB-EP425214EJF
CSB-BP425214EJF
CSB-MP425214EJF
CSB-EP425214EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Putative outer membrane porin BglH (bglH) CSB-YP425215EJF
CSB-EP425215EJF
CSB-BP425215EJF
CSB-MP425215EJF
CSB-EP425215EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 ATP synthase subunit c (atpE), partial CSB-YP425216EJF1
CSB-EP425216EJF1
CSB-BP425216EJF1
CSB-MP425216EJF1
CSB-EP425216EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Putative ECA polymerase (wzyE), partial CSB-YP425217EJF
CSB-EP425217EJF
CSB-BP425217EJF
CSB-MP425217EJF
CSB-EP425217EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP425218EJF
CSB-EP425218EJF
CSB-BP425218EJF
CSB-MP425218EJF
CSB-EP425218EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>