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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O139:H28 Trigger factor (tig) CSB-YP425059EJD
CSB-EP425059EJD
CSB-BP425059EJD
CSB-MP425059EJD
CSB-EP425059EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Adenylate kinase (adk) CSB-YP425060EJD
CSB-EP425060EJD
CSB-BP425060EJD
CSB-MP425060EJD
CSB-EP425060EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Carboxylate-amine ligase YbdK (ybdK) CSB-YP425061EJD
CSB-EP425061EJD
CSB-BP425061EJD
CSB-MP425061EJD
CSB-EP425061EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0250 protein ybeD (ybeD) CSB-YP425062EJD
CSB-EP425062EJD
CSB-BP425062EJD
CSB-MP425062EJD
CSB-EP425062EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Probable rRNA maturation factor YbeY (ybeY) CSB-YP425063EJD
CSB-EP425063EJD
CSB-BP425063EJD
CSB-MP425063EJD
CSB-EP425063EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Endonuclease 8 (nei) CSB-YP425064EJD
CSB-EP425064EJD
CSB-BP425064EJD
CSB-MP425064EJD
CSB-EP425064EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Biotin synthase (bioB) CSB-YP425065EJD
CSB-EP425065EJD
CSB-BP425065EJD
CSB-MP425065EJD
CSB-EP425065EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 DNA protection during starvation protein (dps) CSB-YP425066EJD
CSB-EP425066EJD
CSB-BP425066EJD
CSB-MP425066EJD
CSB-EP425066EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Translation initiation factor IF-1 (infA) CSB-YP425067EJD
CSB-EP425067EJD
CSB-BP425067EJD
CSB-MP425067EJD
CSB-EP425067EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Integration host factor subunit beta (ihfB) CSB-YP425068EJD
CSB-EP425068EJD
CSB-BP425068EJD
CSB-MP425068EJD
CSB-EP425068EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Alkanesulfonate monooxygenase (ssuD) CSB-YP425069EJD
CSB-EP425069EJD
CSB-BP425069EJD
CSB-MP425069EJD
CSB-EP425069EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP425070EJD
CSB-EP425070EJD
CSB-BP425070EJD
CSB-MP425070EJD
CSB-EP425070EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Putative aminoacrylate peracid reductase RutC (rutC) CSB-YP425071EJD
CSB-EP425071EJD
CSB-BP425071EJD
CSB-MP425071EJD
CSB-EP425071EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 N-methyl-L-tryptophan oxidase (solA) CSB-YP425072EJD
CSB-EP425072EJD
CSB-BP425072EJD
CSB-MP425072EJD
CSB-EP425072EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP425073EJD
CSB-EP425073EJD
CSB-BP425073EJD
CSB-MP425073EJD
CSB-EP425073EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0260 protein ycgN (ycgN) CSB-YP425074EJD
CSB-EP425074EJD
CSB-BP425074EJD
CSB-MP425074EJD
CSB-EP425074EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP425075EJD
CSB-EP425075EJD
CSB-BP425075EJD
CSB-MP425075EJD
CSB-EP425075EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0263 protein yciU (yciU) CSB-YP425076EJD
CSB-EP425076EJD
CSB-BP425076EJD
CSB-MP425076EJD
CSB-EP425076EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 GTP cyclohydrolase-2 (ribA) CSB-YP425077EJD
CSB-EP425077EJD
CSB-BP425077EJD
CSB-MP425077EJD
CSB-EP425077EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Tautomerase pptA (pptA) CSB-YP425078EJD
CSB-EP425078EJD
CSB-BP425078EJD
CSB-MP425078EJD
CSB-EP425078EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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