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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Escherichia coli O139:H28 DnaA-homolog protein hda (hda) CSB-YP425100EJD
CSB-EP425100EJD
CSB-BP425100EJD
CSB-MP425100EJD
CSB-EP425100EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP425101EJD
CSB-EP425101EJD
CSB-BP425101EJD
CSB-MP425101EJD
CSB-EP425101EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 3-phenylpropionate/cinnamic acid dioxygenase subunit beta (hcaF) CSB-YP425102EJD
CSB-EP425102EJD
CSB-BP425102EJD
CSB-MP425102EJD
CSB-EP425102EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 DNA repair protein recO (recO) CSB-YP425103EJD
CSB-EP425103EJD
CSB-BP425103EJD
CSB-MP425103EJD
CSB-EP425103EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 tRNA (guanine-N (1)-)-methyltransferase CSB-YP425104EJD
CSB-EP425104EJD
CSB-BP425104EJD
CSB-MP425104EJD
CSB-EP425104EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Protein nrdI (nrdI) CSB-YP425105EJD
CSB-EP425105EJD
CSB-BP425105EJD
CSB-MP425105EJD
CSB-EP425105EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Anaerobic nitric oxide reductase transcription regulator norR (norR) CSB-YP425106EJD
CSB-EP425106EJD
CSB-BP425106EJD
CSB-MP425106EJD
CSB-EP425106EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP425107EJD
CSB-EP425107EJD
CSB-BP425107EJD
CSB-MP425107EJD
CSB-EP425107EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Enolase (eno) CSB-YP425108EJD
CSB-EP425108EJD
CSB-BP425108EJD
CSB-MP425108EJD
CSB-EP425108EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Isopentenyl-diphosphate Delta-isomerase (idi) CSB-YP425109EJD
CSB-EP425109EJD
CSB-BP425109EJD
CSB-MP425109EJD
CSB-EP425109EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Phosphoglycerate kinase (pgk) CSB-YP425110EJD
CSB-EP425110EJD
CSB-BP425110EJD
CSB-MP425110EJD
CSB-EP425110EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Probable Fe (2+)-trafficking protein CSB-YP425111EJD
CSB-EP425111EJD
CSB-BP425111EJD
CSB-MP425111EJD
CSB-EP425111EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Multifunctional CCA protein (cca) CSB-YP425112EJD
CSB-EP425112EJD
CSB-BP425112EJD
CSB-MP425112EJD
CSB-EP425112EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Uronate isomerase (uxaC) CSB-YP425113EJD
CSB-EP425113EJD
CSB-BP425113EJD
CSB-MP425113EJD
CSB-EP425113EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0306 protein yhbP (yhbP) CSB-YP425114EJD
CSB-EP425114EJD
CSB-BP425114EJD
CSB-MP425114EJD
CSB-EP425114EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP425115EJD1
CSB-EP425115EJD1
CSB-BP425115EJD1
CSB-MP425115EJD1
CSB-EP425115EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Arginine repressor (argR) CSB-YP425116EJD
CSB-EP425116EJD
CSB-BP425116EJD
CSB-MP425116EJD
CSB-EP425116EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 50S ribosomal protein L15 (rplO) CSB-YP425117EJD
CSB-EP425117EJD
CSB-BP425117EJD
CSB-MP425117EJD
CSB-EP425117EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 50S ribosomal protein L24 (rplX) CSB-YP425118EJD
CSB-EP425118EJD
CSB-BP425118EJD
CSB-MP425118EJD
CSB-EP425118EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 50S ribosomal protein L22 (rplV) CSB-YP425119EJD
CSB-EP425119EJD
CSB-BP425119EJD
CSB-MP425119EJD
CSB-EP425119EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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