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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Citrobacter koseri DNA mismatch repair protein mutH (mutH) CSB-YP425300CWS
CSB-EP425300CWS
CSB-BP425300CWS
CSB-MP425300CWS
CSB-EP425300CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Chromosome initiation inhibitor (iciA) CSB-YP425301CWS
CSB-EP425301CWS
CSB-BP425301CWS
CSB-MP425301CWS
CSB-EP425301CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Membrane-bound lytic murein transglycosylase C (mltC) CSB-YP425302CWS
CSB-EP425302CWS
CSB-BP425302CWS
CSB-MP425302CWS
CSB-EP425302CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Urease accessory protein UreD (ureD) CSB-YP425303CWS
CSB-EP425303CWS
CSB-BP425303CWS
CSB-MP425303CWS
CSB-EP425303CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Urease accessory protein UreF (ureF) CSB-YP425304CWS
CSB-EP425304CWS
CSB-BP425304CWS
CSB-MP425304CWS
CSB-EP425304CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Putrescine aminotransferase (patA) CSB-YP425305CWS
CSB-EP425305CWS
CSB-BP425305CWS
CSB-MP425305CWS
CSB-EP425305CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Threonine/serine transporter TdcC (tdcC), partial CSB-YP425306CWS
CSB-EP425306CWS
CSB-BP425306CWS
CSB-MP425306CWS
CSB-EP425306CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 30S ribosomal protein S15 (rpsO) CSB-YP425307CWS
CSB-EP425307CWS
CSB-BP425307CWS
CSB-MP425307CWS
CSB-EP425307CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri GTPase obg (obg) CSB-YP425308CWS
CSB-EP425308CWS
CSB-BP425308CWS
CSB-MP425308CWS
CSB-EP425308CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri N-acetylmannosamine kinase (nanK) CSB-YP425309CWS
CSB-EP425309CWS
CSB-BP425309CWS
CSB-MP425309CWS
CSB-EP425309CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Protein AaeX (aaeX), partial CSB-YP425310CWS1
CSB-EP425310CWS1
CSB-BP425310CWS1
CSB-MP425310CWS1
CSB-EP425310CWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Shikimate dehydrogenase (aroE) CSB-YP425311CWS
CSB-EP425311CWS
CSB-BP425311CWS
CSB-MP425311CWS
CSB-EP425311CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 30S ribosomal protein S5 (rpsE) CSB-YP425312CWS
CSB-EP425312CWS
CSB-BP425312CWS
CSB-MP425312CWS
CSB-EP425312CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 50S ribosomal protein L5 (rplE) CSB-YP425313CWS
CSB-EP425313CWS
CSB-BP425313CWS
CSB-MP425313CWS
CSB-EP425313CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 30S ribosomal protein S3 (rpsC) CSB-YP425314CWS
CSB-EP425314CWS
CSB-BP425314CWS
CSB-MP425314CWS
CSB-EP425314CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Glutathione-regulated potassium-efflux system protein KefB (kefB), partial CSB-YP425315CWS
CSB-EP425315CWS
CSB-BP425315CWS
CSB-MP425315CWS
CSB-EP425315CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP425316CWS
CSB-EP425316CWS
CSB-BP425316CWS
CSB-MP425316CWS
CSB-EP425316CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Rhomboid protease glpG (glpG), partial CSB-YP425317CWS1
CSB-EP425317CWS1
CSB-BP425317CWS1
CSB-MP425317CWS1
CSB-EP425317CWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Universal stress protein B (uspB), partial CSB-YP425318CWS1
CSB-EP425318CWS1
CSB-BP425318CWS1
CSB-MP425318CWS1
CSB-EP425318CWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP425319CWS
CSB-EP425319CWS
CSB-BP425319CWS
CSB-MP425319CWS
CSB-EP425319CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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