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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus S-adenosylmethionine synthase (metK) CSB-YP431743PZC
CSB-EP431743PZC
CSB-BP431743PZC
CSB-MP431743PZC
CSB-EP431743PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem I reaction center subunit IV (psaE) CSB-YP431744PZC
CSB-EP431744PZC
CSB-BP431744PZC
CSB-MP431744PZC
CSB-EP431744PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Probable malate:quinone oxidoreductase (mqo) CSB-YP431745PZC
CSB-EP431745PZC
CSB-BP431745PZC
CSB-MP431745PZC
CSB-EP431745PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UPF0234 protein P9211_04811 (P9211_04811) CSB-YP431746PZC
CSB-EP431746PZC
CSB-BP431746PZC
CSB-MP431746PZC
CSB-EP431746PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP431747PZC
CSB-EP431747PZC
CSB-BP431747PZC
CSB-MP431747PZC
CSB-EP431747PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP431748PZC
CSB-EP431748PZC
CSB-BP431748PZC
CSB-MP431748PZC
CSB-EP431748PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis UPF0296 protein Pmob_0099 (Pmob_0099) CSB-YP431749PYI
CSB-EP431749PYI
CSB-BP431749PYI
CSB-MP431749PYI
CSB-EP431749PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Leucine--tRNA ligase (leuS), partial CSB-YP431750PYI
CSB-EP431750PYI
CSB-BP431750PYI
CSB-MP431750PYI
CSB-EP431750PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis SsrA-binding protein (smpB) CSB-YP431751PYI
CSB-EP431751PYI
CSB-BP431751PYI
CSB-MP431751PYI
CSB-EP431751PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis ATP-dependent zinc metalloprotease FtsH 1 (ftsH1), partial CSB-YP431752PYI1
CSB-EP431752PYI1
CSB-BP431752PYI1
CSB-MP431752PYI1
CSB-EP431752PYI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 30S ribosomal protein S13 (rpsM) CSB-YP431753PYI
CSB-EP431753PYI
CSB-BP431753PYI
CSB-MP431753PYI
CSB-EP431753PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Homoserine kinase (thrB) CSB-YP431754PYI
CSB-EP431754PYI
CSB-BP431754PYI
CSB-MP431754PYI
CSB-EP431754PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Phosphate acyltransferase (plsX) CSB-YP431755PYI
CSB-EP431755PYI
CSB-BP431755PYI
CSB-MP431755PYI
CSB-EP431755PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 30S ribosomal protein S18 (rpsR) CSB-YP431756PYI
CSB-EP431756PYI
CSB-BP431756PYI
CSB-MP431756PYI
CSB-EP431756PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 50S ribosomal protein L22 (rplV) CSB-YP431757PYI
CSB-EP431757PYI
CSB-BP431757PYI
CSB-MP431757PYI
CSB-EP431757PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 30S ribosomal protein S9 (rpsI) CSB-YP431758PYI
CSB-EP431758PYI
CSB-BP431758PYI
CSB-MP431758PYI
CSB-EP431758PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Probable rRNA maturation factor (Pmob_0846) CSB-YP431759PYI
CSB-EP431759PYI
CSB-BP431759PYI
CSB-MP431759PYI
CSB-EP431759PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP431760PYI
CSB-EP431760PYI
CSB-BP431760PYI
CSB-MP431760PYI
CSB-EP431760PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP431761PYI
CSB-EP431761PYI
CSB-BP431761PYI
CSB-MP431761PYI
CSB-EP431761PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 30S ribosomal protein S20 (rpsT) CSB-YP431762PYI
CSB-EP431762PYI
CSB-BP431762PYI
CSB-MP431762PYI
CSB-EP431762PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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