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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Delftia acidovorans 30S ribosomal protein S5 (rpsE) CSB-YP431783DHV
CSB-EP431783DHV
CSB-BP431783DHV
CSB-MP431783DHV
CSB-EP431783DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L17 (rplQ) CSB-YP431784DHV
CSB-EP431784DHV
CSB-BP431784DHV
CSB-MP431784DHV
CSB-EP431784DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Dihydroorotase (pyrC) CSB-YP431785DHV
CSB-EP431785DHV
CSB-BP431785DHV
CSB-MP431785DHV
CSB-EP431785DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Urease subunit alpha (ureC), partial CSB-YP431786DHV
CSB-EP431786DHV
CSB-BP431786DHV
CSB-MP431786DHV
CSB-EP431786DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP431787DHV
CSB-EP431787DHV
CSB-BP431787DHV
CSB-MP431787DHV
CSB-EP431787DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP431788DHV
CSB-EP431788DHV
CSB-BP431788DHV
CSB-MP431788DHV
CSB-EP431788DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans N utilization substance protein B homolog (nusB) CSB-YP431789DHV
CSB-EP431789DHV
CSB-BP431789DHV
CSB-MP431789DHV
CSB-EP431789DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Adenylate kinase (adk) CSB-YP431790DHV
CSB-EP431790DHV
CSB-BP431790DHV
CSB-MP431790DHV
CSB-EP431790DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP431791DHV
CSB-EP431791DHV
CSB-BP431791DHV
CSB-MP431791DHV
CSB-EP431791DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP431792DHV
CSB-EP431792DHV
CSB-BP431792DHV
CSB-MP431792DHV
CSB-EP431792DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans UPF0301 protein Daci_1578 (Daci_1578) CSB-YP431793DHV
CSB-EP431793DHV
CSB-BP431793DHV
CSB-MP431793DHV
CSB-EP431793DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Inositol 2-dehydrogenase (iolG) CSB-YP431794DHV
CSB-EP431794DHV
CSB-BP431794DHV
CSB-MP431794DHV
CSB-EP431794DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans UPF0176 protein Daci_4043 (Daci_4043) CSB-YP431795DHV
CSB-EP431795DHV
CSB-BP431795DHV
CSB-MP431795DHV
CSB-EP431795DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Potassium-transporting ATPase A chain (kdpA), partial CSB-YP431796DHV
CSB-EP431796DHV
CSB-BP431796DHV
CSB-MP431796DHV
CSB-EP431796DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Trigger factor (tig) CSB-YP431797DHV
CSB-EP431797DHV
CSB-BP431797DHV
CSB-MP431797DHV
CSB-EP431797DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP431798DHV
CSB-EP431798DHV
CSB-BP431798DHV
CSB-MP431798DHV
CSB-EP431798DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Release factor glutamine methyltransferase (prmC) CSB-YP431799AYS
CSB-EP431799AYS
CSB-BP431799AYS
CSB-MP431799AYS
CSB-EP431799AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens 30S ribosomal protein S14 (rpsN) CSB-YP431800AYS
CSB-EP431800AYS
CSB-BP431800AYS
CSB-MP431800AYS
CSB-EP431800AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP431801AYS
CSB-EP431801AYS
CSB-BP431801AYS
CSB-MP431801AYS
CSB-EP431801AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryzias latipes Tudor domain-containing protein 1 (tdrd1), partial CSB-YP431802OFH
CSB-EP431802OFH
CSB-BP431802OFH
CSB-MP431802OFH
CSB-EP431802OFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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