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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus Protein grpE (grpE) CSB-YP431722PZC
CSB-EP431722PZC
CSB-BP431722PZC
CSB-MP431722PZC
CSB-EP431722PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP431723PZC
CSB-EP431723PZC
CSB-BP431723PZC
CSB-MP431723PZC
CSB-EP431723PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UvrABC system protein C (uvrC), partial CSB-YP431724PZC
CSB-EP431724PZC
CSB-BP431724PZC
CSB-MP431724PZC
CSB-EP431724PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP431725PZC
CSB-EP431725PZC
CSB-BP431725PZC
CSB-MP431725PZC
CSB-EP431725PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP431726PZC
CSB-EP431726PZC
CSB-BP431726PZC
CSB-MP431726PZC
CSB-EP431726PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus PKHD-type hydroxylase P9211_12561 (P9211_12561) CSB-YP431727PZC
CSB-EP431727PZC
CSB-BP431727PZC
CSB-MP431727PZC
CSB-EP431727PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 5'-nucleotidase surE (surE) CSB-YP431728PZC
CSB-EP431728PZC
CSB-BP431728PZC
CSB-MP431728PZC
CSB-EP431728PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP431729PZC
CSB-EP431729PZC
CSB-BP431729PZC
CSB-MP431729PZC
CSB-EP431729PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP431731PZC
CSB-EP431731PZC
CSB-BP431731PZC
CSB-MP431731PZC
CSB-EP431731PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S10 (rpsJ) CSB-YP431732PZC
CSB-EP431732PZC
CSB-BP431732PZC
CSB-MP431732PZC
CSB-EP431732PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L5 (rplE) CSB-YP431733PZC
CSB-EP431733PZC
CSB-BP431733PZC
CSB-MP431733PZC
CSB-EP431733PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S19 (rpsS) CSB-YP431734PZC
CSB-EP431734PZC
CSB-BP431734PZC
CSB-MP431734PZC
CSB-EP431734PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP431735PZC
CSB-EP431735PZC
CSB-BP431735PZC
CSB-MP431735PZC
CSB-EP431735PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit H CSB-YP431736PZC
CSB-EP431736PZC
CSB-BP431736PZC
CSB-MP431736PZC
CSB-EP431736PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphomethylpyrimidine synthase (thiC) CSB-YP431737PZC
CSB-EP431737PZC
CSB-BP431737PZC
CSB-MP431737PZC
CSB-EP431737PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Dihydrodipicolinate synthase (dapA) CSB-YP431738PZC
CSB-EP431738PZC
CSB-BP431738PZC
CSB-MP431738PZC
CSB-EP431738PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L9 (rplI) CSB-YP431739PZC
CSB-EP431739PZC
CSB-BP431739PZC
CSB-MP431739PZC
CSB-EP431739PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Arginine--tRNA ligase (argS), partial CSB-YP431740PZC
CSB-EP431740PZC
CSB-BP431740PZC
CSB-MP431740PZC
CSB-EP431740PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L11 (rplK) CSB-YP431741PZC
CSB-EP431741PZC
CSB-BP431741PZC
CSB-MP431741PZC
CSB-EP431741PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP431742PZC
CSB-EP431742PZC
CSB-BP431742PZC
CSB-MP431742PZC
CSB-EP431742PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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