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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lottia gigantea EGF-like domain-containing protein 1 CSB-YP453177LQP
CSB-EP453177LQP
CSB-BP453177LQP
CSB-MP453177LQP
CSB-EP453177LQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lottia gigantea Aspartate and serine-rich protein CSB-YP453178LQP
CSB-EP453178LQP
CSB-BP453178LQP
CSB-MP453178LQP
CSB-EP453178LQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 60 kDa chaperonin (groL), partial CSB-YP453179WBO
CSB-EP453179WBO
CSB-BP453179WBO
CSB-MP453179WBO
CSB-EP453179WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 30S ribosomal protein S7 (rpsG) CSB-YP453180WBO
CSB-EP453180WBO
CSB-BP453180WBO
CSB-MP453180WBO
CSB-EP453180WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP453181WBO
CSB-EP453181WBO
CSB-BP453181WBO
CSB-MP453181WBO
CSB-EP453181WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens ATP synthase subunit c (atpE), partial CSB-YP453182WBO1
CSB-EP453182WBO1
CSB-BP453182WBO1
CSB-MP453182WBO1
CSB-EP453182WBO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 50S ribosomal protein L9 (rplI) CSB-YP453183WBO
CSB-EP453183WBO
CSB-BP453183WBO
CSB-MP453183WBO
CSB-EP453183WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens UPF0176 protein WP0716 (WP0716) CSB-YP453184WBO
CSB-EP453184WBO
CSB-BP453184WBO
CSB-MP453184WBO
CSB-EP453184WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Dihydrodipicolinate synthase (dapA) CSB-YP453185WBO
CSB-EP453185WBO
CSB-BP453185WBO
CSB-MP453185WBO
CSB-EP453185WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP453186WBO
CSB-EP453186WBO
CSB-BP453186WBO
CSB-MP453186WBO
CSB-EP453186WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP453187WBO
CSB-EP453187WBO
CSB-BP453187WBO
CSB-MP453187WBO
CSB-EP453187WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP453188WBO
CSB-EP453188WBO
CSB-BP453188WBO
CSB-MP453188WBO
CSB-EP453188WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP453189WBO
CSB-EP453189WBO
CSB-BP453189WBO
CSB-MP453189WBO
CSB-EP453189WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Protein-export protein secB (secB) CSB-YP453190WBO
CSB-EP453190WBO
CSB-BP453190WBO
CSB-MP453190WBO
CSB-EP453190WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Phosphopantetheine adenylyltransferase (coaD) CSB-YP453191WBO
CSB-EP453191WBO
CSB-BP453191WBO
CSB-MP453191WBO
CSB-EP453191WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 50S ribosomal protein L22 (rplV) CSB-YP453192WBO
CSB-EP453192WBO
CSB-BP453192WBO
CSB-MP453192WBO
CSB-EP453192WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Dihydrodipicolinate reductase (dapB) CSB-YP453193WBO
CSB-EP453193WBO
CSB-BP453193WBO
CSB-MP453193WBO
CSB-EP453193WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Deoxycytidine triphosphate deaminase (dcd) CSB-YP453194WBO
CSB-EP453194WBO
CSB-BP453194WBO
CSB-MP453194WBO
CSB-EP453194WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 30S ribosomal protein S4 (rpsD) CSB-YP453195WBO
CSB-EP453195WBO
CSB-BP453195WBO
CSB-MP453195WBO
CSB-EP453195WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Putative phosphotransferase WP0486 (WP0486) CSB-YP453196WBO
CSB-EP453196WBO
CSB-BP453196WBO
CSB-MP453196WBO
CSB-EP453196WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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