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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Erwinia tasmaniensis Alanine racemase (alr) CSB-YP453137ENB
CSB-EP453137ENB
CSB-BP453137ENB
CSB-MP453137ENB
CSB-EP453137ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis tRNA (mo5U34)-methyltransferase CSB-YP453138ENB
CSB-EP453138ENB
CSB-BP453138ENB
CSB-MP453138ENB
CSB-EP453138ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Potassium-transporting ATPase B chain (kdpB), partial CSB-YP453139ENB1
CSB-EP453139ENB1
CSB-BP453139ENB1
CSB-MP453139ENB1
CSB-EP453139ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 33 kDa chaperonin (hslO) CSB-YP453140ENB
CSB-EP453140ENB
CSB-BP453140ENB
CSB-MP453140ENB
CSB-EP453140ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Glutathione-regulated potassium-efflux system protein KefB (kefB), partial CSB-YP453141ENB
CSB-EP453141ENB
CSB-BP453141ENB
CSB-MP453141ENB
CSB-EP453141ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 30S ribosomal protein S19 (rpsS) CSB-YP453142ENB
CSB-EP453142ENB
CSB-BP453142ENB
CSB-MP453142ENB
CSB-EP453142ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 50S ribosomal protein L30 (rpmD) CSB-YP453143ENB
CSB-EP453143ENB
CSB-BP453143ENB
CSB-MP453143ENB
CSB-EP453143ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP453144ENB
CSB-EP453144ENB
CSB-BP453144ENB
CSB-MP453144ENB
CSB-EP453144ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP453145ENB
CSB-EP453145ENB
CSB-BP453145ENB
CSB-MP453145ENB
CSB-EP453145ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Tryptophan synthase beta chain (trpB) CSB-YP453146ENB
CSB-EP453146ENB
CSB-BP453146ENB
CSB-MP453146ENB
CSB-EP453146ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP453147ENB
CSB-EP453147ENB
CSB-BP453147ENB
CSB-MP453147ENB
CSB-EP453147ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP453148ENB
CSB-EP453148ENB
CSB-BP453148ENB
CSB-MP453148ENB
CSB-EP453148ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis High osmolarity signaling protein sho1 (sho1), partial CSB-YP453149FHT1
CSB-EP453149FHT1
CSB-BP453149FHT1
CSB-MP453149FHT1
CSB-EP453149FHT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis Tethering factor for nuclear proteasome sts1 (sts1) CSB-YP453150FHT
CSB-EP453150FHT
CSB-BP453150FHT
CSB-MP453150FHT
CSB-EP453150FHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis Probable zinc metalloprotease PTRG_04977 (PTRG_04977) CSB-YP453151FHT
CSB-EP453151FHT
CSB-BP453151FHT
CSB-MP453151FHT
CSB-EP453151FHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis Bifunctional lycopene cyclase/phytoene synthase (PTRG_07366), partial CSB-YP453152FHT1
CSB-EP453152FHT1
CSB-BP453152FHT1
CSB-MP453152FHT1
CSB-EP453152FHT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis Altered inheritance of mitochondria protein 31, mitochondrial (aim31), partial CSB-YP453153FHT1
CSB-EP453153FHT1
CSB-BP453153FHT1
CSB-MP453153FHT1
CSB-EP453153FHT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis Carboxypeptidase Y homolog A (cpyA) CSB-YP453154FHT
CSB-EP453154FHT
CSB-BP453154FHT
CSB-MP453154FHT
CSB-EP453154FHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis Amino-acid acetyltransferase, mitochondrial (arg2), partial CSB-YP453155FHT
CSB-EP453155FHT
CSB-BP453155FHT
CSB-MP453155FHT
CSB-EP453155FHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oedogonium cardiacum Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP453156ODX1
CSB-EP453156ODX1
CSB-BP453156ODX1
CSB-MP453156ODX1
CSB-EP453156ODX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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