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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Francisella tularensis subsp. mediasiatica 50S ribosomal protein L19 (rplS) CSB-YP454738FFF
CSB-EP454738FFF
CSB-BP454738FFF
CSB-MP454738FFF
CSB-EP454738FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Proline--tRNA ligase (proS), partial CSB-YP454739FFF
CSB-EP454739FFF
CSB-BP454739FFF
CSB-MP454739FFF
CSB-EP454739FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica tRNA (Ile)-lysidine synthase CSB-YP454740FFF
CSB-EP454740FFF
CSB-BP454740FFF
CSB-MP454740FFF
CSB-EP454740FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Shikimate kinase (aroK) CSB-YP454741FFF
CSB-EP454741FFF
CSB-BP454741FFF
CSB-MP454741FFF
CSB-EP454741FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica 30S ribosomal protein S6 (rpsF) CSB-YP454742FFF
CSB-EP454742FFF
CSB-BP454742FFF
CSB-MP454742FFF
CSB-EP454742FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Deoxycytidine triphosphate deaminase (dcd) CSB-YP454743FFF
CSB-EP454743FFF
CSB-BP454743FFF
CSB-MP454743FFF
CSB-EP454743FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP454744FFF
CSB-EP454744FFF
CSB-BP454744FFF
CSB-MP454744FFF
CSB-EP454744FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP454745FFF
CSB-EP454745FFF
CSB-BP454745FFF
CSB-MP454745FFF
CSB-EP454745FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP454746FFF
CSB-EP454746FFF
CSB-BP454746FFF
CSB-MP454746FFF
CSB-EP454746FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Alanine--tRNA ligase (alaS), partial CSB-YP454747FFF
CSB-EP454747FFF
CSB-BP454747FFF
CSB-MP454747FFF
CSB-EP454747FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP454748XBC
CSB-EP454748XBC
CSB-BP454748XBC
CSB-MP454748XBC
CSB-EP454748XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP454749XBC
CSB-EP454749XBC
CSB-BP454749XBC
CSB-MP454749XBC
CSB-EP454749XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP454750XBC
CSB-EP454750XBC
CSB-BP454750XBC
CSB-MP454750XBC
CSB-EP454750XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP454751XBC
CSB-EP454751XBC
CSB-BP454751XBC
CSB-MP454751XBC
CSB-EP454751XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae ATP phosphoribosyltransferase (hisG) CSB-YP454752XBC
CSB-EP454752XBC
CSB-BP454752XBC
CSB-MP454752XBC
CSB-EP454752XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Indole-3-glycerol phosphate synthase (trpC) CSB-YP454753XBC
CSB-EP454753XBC
CSB-BP454753XBC
CSB-MP454753XBC
CSB-EP454753XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 30S ribosomal protein S21 (rpsU) CSB-YP454754XBC
CSB-EP454754XBC
CSB-BP454754XBC
CSB-MP454754XBC
CSB-EP454754XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Integration host factor subunit beta (ihfB) CSB-YP454755XBC
CSB-EP454755XBC
CSB-BP454755XBC
CSB-MP454755XBC
CSB-EP454755XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP454756XBC
CSB-EP454756XBC
CSB-BP454756XBC
CSB-MP454756XBC
CSB-EP454756XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP454757XBC
CSB-EP454757XBC
CSB-BP454757XBC
CSB-MP454757XBC
CSB-EP454757XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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