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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ralstonia pickettii Chaperone protein DnaJ (dnaJ) CSB-YP454938RJI
CSB-EP454938RJI
CSB-BP454938RJI
CSB-MP454938RJI
CSB-EP454938RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Dihydrodipicolinate reductase (dapB) CSB-YP454939RJI
CSB-EP454939RJI
CSB-BP454939RJI
CSB-MP454939RJI
CSB-EP454939RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii RNA pyrophosphohydrolase (rppH) CSB-YP454940RJI
CSB-EP454940RJI
CSB-BP454940RJI
CSB-MP454940RJI
CSB-EP454940RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii UPF0289 protein Rpic_3074 (Rpic_3074) CSB-YP454941RJI
CSB-EP454941RJI
CSB-BP454941RJI
CSB-MP454941RJI
CSB-EP454941RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Anthranilate phosphoribosyltransferase (trpD) CSB-YP454942RJI
CSB-EP454942RJI
CSB-BP454942RJI
CSB-MP454942RJI
CSB-EP454942RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP454943RJI
CSB-EP454943RJI
CSB-BP454943RJI
CSB-MP454943RJI
CSB-EP454943RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 50S ribosomal protein L17 (rplQ) CSB-YP454944RJI
CSB-EP454944RJI
CSB-BP454944RJI
CSB-MP454944RJI
CSB-EP454944RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 50S ribosomal protein L29 (rpmC) CSB-YP454945RJI
CSB-EP454945RJI
CSB-BP454945RJI
CSB-MP454945RJI
CSB-EP454945RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 50S ribosomal protein L4 (rplD) CSB-YP454946RJI
CSB-EP454946RJI
CSB-BP454946RJI
CSB-MP454946RJI
CSB-EP454946RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Outer-membrane lipoprotein lolB (lolB) CSB-YP454947RJI
CSB-EP454947RJI
CSB-BP454947RJI
CSB-MP454947RJI
CSB-EP454947RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii HPr kinase/phosphorylase (hprK) CSB-YP454948RJI
CSB-EP454948RJI
CSB-BP454948RJI
CSB-MP454948RJI
CSB-EP454948RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP454949RJI
CSB-EP454949RJI
CSB-BP454949RJI
CSB-MP454949RJI
CSB-EP454949RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Putative iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP454950RJI
CSB-EP454950RJI
CSB-BP454950RJI
CSB-MP454950RJI
CSB-EP454950RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP454951RJI
CSB-EP454951RJI
CSB-BP454951RJI
CSB-MP454951RJI
CSB-EP454951RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Phosphoglucosamine mutase (glmM) CSB-YP454952RJI
CSB-EP454952RJI
CSB-BP454952RJI
CSB-MP454952RJI
CSB-EP454952RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Potassium-transporting ATPase A chain (kdpA), partial CSB-YP454953RJI
CSB-EP454953RJI
CSB-BP454953RJI
CSB-MP454953RJI
CSB-EP454953RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Probable L-aspartate dehydrogenase (nadX) CSB-YP454954RJI
CSB-EP454954RJI
CSB-BP454954RJI
CSB-MP454954RJI
CSB-EP454954RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Phosphoglycerate kinase (pgk) CSB-YP454955AZF
CSB-EP454955AZF
CSB-BP454955AZF
CSB-MP454955AZF
CSB-EP454955AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Glycosyl hydrolase family 109 protein 1 (Amuc_0017) CSB-YP454956AZF
CSB-EP454956AZF
CSB-BP454956AZF
CSB-MP454956AZF
CSB-EP454956AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila GTP cyclohydrolase folE2 (folE2) CSB-YP454957AZF
CSB-EP454957AZF
CSB-BP454957AZF
CSB-MP454957AZF
CSB-EP454957AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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